- This product was discontinued on January 01, 2013
Genenase™ I is a variant of subtilisin BPN´ that has been engineered to have increased specificity by substituting amino acids in its active site (1,2). When designing fusion proteins for cleavage with Genenase I, we recommend the site Pro-Gly-Ala-Ala-His-Tyr. Genenase I will cleave at other histidine residues depending on the surrounding amino acids and the 3-dimensional conformation of the protein. Genenase I cleaves His-Tyr-Glu and His-Tyr-Asp slowly, but will not cleave His-Tyr-Pro or His-Tyr-Ile (2).
Product SourceIsolated from a Bacillus subtilis strain carrying the protease gene derived from Bacillus amyloliquefaciens.
SpecificityGenenase I cleaves His-Tyr-Glu and His-Tyr-Asp slowly, but will not cleave His-Tyr-Pro or His-Tyr-Ile (2).
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