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  • ColorPlus™ Prestained Protein Ladder, Broad Range (10-230 kDa)

    This product is replaced by P7712.
    • This product was discontinued on 06/13/2014
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    Discontinued Products

    Description

    ColorPlus™ Prestained Protein Ladder, Broad Range (10-230 kDa) is a mixture of highly purified recombinant proteins covalently coupled to a blue dye that resolves into 12 sharp bands when electrophoresed. The protein concentrations are carefully balanced for even intensity. Both the orange 80 kDa and the green 25 kDa bands serve as the reference indicators. The covalent coupling of the dye to the proteins affects their electrophoretic behavior on SDS-PAGE gels relative to unstained proteins (1). The apparent molecular weights of the ColorPlus Prestained Protein Ladder bands were determined on Invitrogen Novex 10–20% Tris-glycine SDS-PAGE gels (1,2) by comparison to the Protein Ladder (NEB #P7703). The ColorPlus Prestained Protein Ladder is ideally suited for use in SDS-PAGE and western blotting applications. It allows continuous monitoring of protein separations during electrophoresis and also provides a quick and easy way to assess blotting efficiency (3).

    The ColorPlus Prestained Protein Ladder is designed for use with Tris-glycine gels.

    P7711_thumb

    10-20% Tris-glycine SDS-PAGE gel.

    Properties and Usage

    Storage Temperature

    -20°C

    Storage Conditions

    70 mM Tris-HCl
    33 mM NaCl
    40 mM DTT
    1 mM Na2EDTA
    30% Glycerol
    2% SDS
    pH 6.8 @ 25°C

    Notes

    1. To maximize shelf-life, the ColorPlus Prestained Protein Ladder should be boiled for 3-5 minutes upon receipt and aliquotted into single-use tubes. Store at -20°C. Guaranteed stable for 1 year when properly stored.
    2. P7711 is not recommended for use on Tris-Tricine or Bis-Tris (with MES buffer) gels.
    3. Recommended load; 5µl for western blotting and 10µl for SDS-PAGE.

    References

    1. Laemmli, U.K. (1970). Nature. 227, 680.
    2. Sambrook, J., Fritsch, E.F. and Maniatis, T. (2001). Molecular Cloning: A Laboratory Manual, (3rd ed.). Cold Spring Harbor: Cold Spring Harbor Laboratory.
    3. Ma, D. and Xu, M.Q. New England Biolabs, Inc.

    FAQs

    1. I noticed that the molecular weights I am observing are slightly different than those reported. Can you explain?

    Tech Tips

    Prestained proteins carry a charge because of the dye molecules bound to the protein. Because of this, any changes in the running conditions will alter their migration. These products should not be used for accurate molecular weight determination. The unstained markers and ladders are better for that. Prestained are best designed for western blot applications.

    Protocols

    1. Preparing and Loading Protein Ladders (P7711)
    2. Using NEB Protein Markers & Ladders

    Datacards

    The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.

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    Safety Data Sheet

    The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

    Datacards

    The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.