ColorPlus™ Prestained Protein Marker, Broad Range (7-175 kDa)

This product is replaced by P7712.

Catalog # P7709 was discontinued on June 13, 2014 ;

Product Information
ColorPlus™ Prestained Protein Marker, Broad Range includes a mixture of purified proteins covalently coupled to colored dye that resolves to 8 bands of even intensity, including one orange and one green reference band for easy orientation when electrophoresed (1). The covalent coupling of the dye to the proteins affects their electrophoretic behavior in SDS-PAGE gels relative to unstained proteins (1). For precise molecular weight determinations, use NEB's unstained Protein Marker, Broad Range (NEB #P7702) or Protein Ladder (NEB #P7703) in addition to the prestained marker.

Product Categories:

Discontinued Products
- Properties & Usage
  
  Storage Temperature
  -20°C
  Storage Conditions
  
  63.5 mM Tris-HCl
  50 mM NaCl
  40 mM DTT
  1 mM Na₂EDTA
  10% Glycerol
  0.01% Phenol red
  2% SDS
  pH 7.0 @ 25°C
- Related Products
  Companion Products
  
  Blue Protein Loading Dye
- Product Notes
  Recommended load for SDS-PAGE Analysis is 15 μl, while 6 μl is recommended for Western blot analysis.
  
  Apparent molecular weights of every band are determined on Invitrogen Novex 10-20% Tris-glycine SDS PAGE gels using NEB's Protein Ladder (NEB #P7703)..
- References
  Laemmli, U.K. (1970). Nature. 227, 680.
  
  Sambrook, J., Fritsch, E.F. and Maniatis, T. Molecular Cloning: A Laboratory Manual. (2nd ed.), Cold Spring Harbor Laboratory Press.
Protocols, Manuals & Usage
- Protocols
  Suggested Protocol for Loading a Sample (P7709)
  
  Suggested Protocol for Loading the Unstained Protein Ladder, Broad Range (P7703)
- Datacards
  The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at [email protected] or fill out the Technical Support Form for appropriate document.)
  
  P7709Datasheet-Lot0221206
  
  P7709Datasheet-Lot0231211
  
  P7709Datasheet-Lot0241303
  
  P7709Datasheet-Lot0251307
  
  P7709Datasheet-Lot0251308
  
  P7709Datasheet-Lot0261312
FAQs & Troubleshooting
- FAQs
  Is the ColorPlus Prestained Protein Marker stable at room temperature?
  
  After repeated freeze/thaws, I have noticed a fading or speading out of the low molecular weight bands in the ColorPlus Prestained Protein Marker. Is there any way to extend the shelf-life of this product?
  
  Why do the apparent molecular weight values for the prestained protein markers appear to be different in different gel types?
- Tech Tips
  
  Prestained proteins carry a charge because of the dye molecules bound to the protein. Because of this, any changes in the running conditions will alter their migration. These products should not be used for accurate molecular weight determination. The unstained markers and ladders are better for that. Prestained are best designed for western blot applications.
Quality, Safety & Legal
- Quality Control Assays
  
  Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
- Safety Data Sheets
  The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.
- Datacards
  The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at [email protected] or fill out the Technical Support Form for appropriate document.)
  
  P7709Datasheet-Lot0221206
  
  P7709Datasheet-Lot0231211
  
  P7709Datasheet-Lot0241303
  
  P7709Datasheet-Lot0251307
  
  P7709Datasheet-Lot0251308
  
  P7709Datasheet-Lot0261312
- Legal and Disclaimers
  
  This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).
  
  While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.
  
  For more information about commercial rights, please contact NEB's Global Business Development team at [email protected].
  
  This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
  
  New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.