Storage Temperature

-20°C

Storage Conditions

63.5 mM Tris-HCl
50 mM NaCl
40 mM DTT
1 mM Na₂EDTA
10% Glycerol
0.01% Phenol red
2% SDS
pH 7.0 @ 25°C

Companion Products

• Blue Loading Buffer Pack

1. Recommended load for SDS-PAGE Analysis is 15 μl, while 6 μl is recommended for Western blot analysis.
2. Apparent molecular weights of every band are determined on Invitrogen Novex 10-20% Tris-glycine SDS PAGE gels using NEB's Protein Ladder (NEB #P7703)..

1. Laemmli, U.K. (1970). Nature. 227, 680.
2. Sambrook, J., Fritsch, E.F. and Maniatis, T. Molecular Cloning: A Laboratory Manual. (2nd ed.), Cold Spring Harbor Laboratory Press.

1. Is the ColorPlus Prestained Protein Marker stable at room temperature?
2. After repeated freeze/thaws, I have noticed a fading or speading out of the low molecular weight bands in the ColorPlus Prestained Protein Marker. Is there any way to extend the shelf-life of this product?
3. Why do the apparent molecular weight values for the prestained protein markers appear to be different in different gel types?

1. Suggested Protocol for Loading a Sample (P7709)
2. Suggested Protocol for Loading the Unstained Protein Ladder, Broad Range (P7703)

• NEBioCalculator

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• ColorPlus™ Prestained Protein Marker, Broad Range (7-175 kDa)

This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).

While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.

For more information about commercial rights, please contact NEB's Global Business Development team at gbd@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.