The Unstained Protein Standard, Broad Range is a mixture of 12 recombinant, highly purified proteins, which resolve into clearly identifiable sharp bands from 10-200 kDa when analyzed by SDS-PAGE and stained with a commercially available, ready-to-use protein stain. The 25 and 85 kDa bands have increased intensity and serve as reference indicators.
The Unstained Protein Standard, Broad Range is a mixture of 12 recombinant, highly purified proteins, which resolve into clearly identifiable sharp bands from 10-200 kDa when analyzed by SDS-PAGE and stained with Coomassie Brilliant Blue R-250 (1,2) or other commercially available, ready-to-use protein stains. This standard allows accurate molecular weight determination when performing SDS-PAGE analysis. The 25 and 85 kDa bands have increased intensity and serve as reference indicators. The apparent molecular weights of the Unstained Protein Standard, Broad Range were determined on Invitrogen Novex 10-20% Tris-glycine gels by comparison to NEB’s previous unstained protein Ladder (NEB # P7703 ).
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