Unstained Protein Standard, Broad Range (10-200 kDa)

This product has been discontinued and replaced with Unstained Protein Standard, Broad Range (10-200 kDa) (NEB #P7717).

Catalog # P7704 was discontinued on May 01, 2019

The Unstained Protein Standard, Broad Range is a mixture of 12 recombinant, highly purified proteins, which resolve into clearly identifiable sharp bands from 10-200 kDa when analyzed by SDS-PAGE and stained with a commercially available, ready-to-use protein stain. The 25 and 85 kDa bands have increased intensity and serve as reference indicators.

Allows accurate molecular weight determination when performing SDS-PAGE analysis.
Direct loading, additional loading buffer and heat incubation not required
Recombinant proteins with no detectable protease contaminating activities
Optimal stability for up to 24 months

Storage Temperature: -20°C

Product Information
The Unstained Protein Standard, Broad Range is a mixture of 12 recombinant, highly purified proteins, which resolve into clearly identifiable sharp bands from 10-200 kDa when analyzed by SDS-PAGE and stained with Coomassie Brilliant Blue R-250 (1,2) or other commercially available, ready-to-use protein stains. This standard allows accurate molecular weight determination when performing SDS-PAGE analysis. The 25 and 85 kDa bands have increased intensity and serve as reference indicators. The apparent molecular weights of the Unstained Protein Standard, Broad Range were determined on Invitrogen Novex 10-20% Tris-glycine gels by comparison to NEB’s previous unstained protein Ladder (NEB # P7703 ).

Product Categories:

Discontinued Products
- Properties & Usage
  Storage Buffer
  
  20 mM Tris-phosphate
  2% SDS
  0.2 mM DTT
  3.6 M Urea
  15% Glycerol
  0.01% bromophenol blue
  pH 7.5 @ 25°C
  
  Long Term Storage
  
  Shelf Life 2 years
- Product Notes
  To maximize shelf life, store at -20°C. Guaranteed stable for 2 years when properly stored.
  
  To ensure uniform mobility, load an equal volume of 1X Reducing SDS Loading Buffer into any unused wells.
  
  It is not necessary to pre-heat the unstained protein standard prior to loading on a gel.
  
  Recommended mini-gel load: 3 μl.
  
  Molecular weight determination by SDS-PAGE is a dependable method. However, an unknown protein’s molecular weight should be obtained by mass spectrometry if a precise molecular weight determination is required. Mass spectrometry has a higher degree of accuracy because each amino acid of a protein is analyzed.
- References
  Laemmli, U.K. (1970). Nature. 227, 680.
  
  Sambrook, J., Fritsch, E.F. and Maniatis, T. (2001). Molecular Cloning: A Laboratory Manual. (3rd ed.), Cold Spring Harbor: Cold Spring Harbor Laboratory Press.
Protocols, Manuals & Usage
- Protocols
  Suggested Protocol for Loading the Unstained Protein Standard (P7704)
FAQs & Troubleshooting
- FAQs
  Can the Unstained Protein Standard, Broad Range be used as a precise standard for determining the exact molecular weight of a protein of interest?
  
  Is the Unstained Protein Standard, Broad Range compatible with various methods of staining, such as SYPRO® Ruby Protein Gel Stain or Silver Staining?
  
  I accidentally left my Protein Standard out at room temperature for a number of days. Is it still okay to use?
Citations & Technical Literature
- Citations
  
  Product Citation Tool
  
  Powered by Bioz See more details on Bioz
Quality, Safety & Legal
- Quality Control Assays
  
  Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
- Specifications & Change Notifications
  Specifications
  The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
  
  P7704S_L_v1
- Certificate of Analysis
  The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
  
  P7704S_L_v1_0011608
  
  P7704S_L_v1_0011612
  
  P7704S_L_v1_0011708
  
  P7704L_v1_10015473
  
  P7704L_v1_10007547
  
  P7704S_v1_10007548
  
  P7704S_v1_10018029
  
  P7704S_v1_10026486
  
  P7704S_v1_10032263
  
  P7704L_v1_10032266
- Safety Data Sheets
  The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.
  
  Unstained Protein Standard, Broad Range (10-200 kDa)
- Legal and Disclaimers
  
  This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).
  
  While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.
  
  For more information about commercial rights, please contact NEB's Global Business Development team at gbd@neb.com.
  
  This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
  
  New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.
  Trademarks
  NEW ENGLAND BIOLABS^® is a registered trademark of New England Biolabs, Inc.
  
  © Copyright 2016, New England Biolabs, Inc; all rights reserved.