Unstained Protein Ladder is a mixture of 12 recombinant, highly purified proteins, which resolve into clearly identifiable sharp bands from 10-250 kDa when analyzed by SDS-PAGE and stained with Coomassie Brilliant Blue R-250 (1,2). This ladder is intended for use as a precise size standard when performing SDS-PAGE to calculate the molecular weight of a protein of interest. The 25 and 80 kDa bands have triple the intensity of the other proteins and serve as reference indicators. The molecular weights of the Protein Ladder are confirmed by sequencing, mass spectrometry and migration in a Laemmli SDS-Page Tris-Glycine system (3).
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