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  • Protein Marker, Broad Range (2-212 kDa)

    Description

    Protein Marker, Broad Range is a mixture of purified proteins with known amino acid sequences. They are resolved to 13 sharp bands when analyzed by SDS-PAGE (Tris-Glycine) and stained with Coomassie Blue R-250 (1). Two bands (BSA, MW 66.4 kDa and Triosephosphate isomerase, MW 26.6 kDa) are at double intensity to serve as reference points.

    P7702_thumb

    10-20% SDS-PAGE
    Insulin chains are unresolved by SDS-PAGE (Tris-Glycine).

    Properties and Usage

    Storage Temperature

    -20°C

    Storage Conditions

    70 mM Tris-HCl
    33 mM NaCl
    40 mM DTT
    1 mM Na2EDTA
    10% Glycerol
    0.01% bromophenol blue
    2% SDS
    pH 6.8 @ 25°C

    Notes

    1. MBP2*= maltose-binding protein. MBP-β-galactosidase = fusion of MBP and β-galactosidase.Trypsin inhibitor (soybean) is a mixture of three isoforms: A-20,094 Da; B-20,040 Da; C-20,167 Da. These isoforms migrate as one unresolved band using Tris-Glycine gels and as a doublet using Tris-Tricine gel. [Schagger, H. and Von Jagow, G. (1987) Anal. Biochem. 166, 368-379].
    2. Recommended Load 7µl.

    References

    1. Laemmli, U.K. (1970). Nature. 227, 680.
    2. Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989). Molecular Cloning: A Laboratory Manual, (2nd ed.), Cold Spring Harbor: Cold Spring Harbor Laboratory Press.

    Supporting Documents

    Material Safety Datasheets

    The following is a list of Material Safety Data Sheets (MSDS) that apply to this product to help you use it safely. The following file naming structure is used to name these document files: [Product Name] MSDS. For international versions please contact us at info@neb.com.

    Datacards

    The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.
    1. Can I use your BSA as a molecular weight marker?
    1. Suggested Protocol for Loading a Sample (P7702)
    2. Using NEB Protein Markers & Ladders