p42 MAP Kinase (MAPK)
- This product was discontinued on January 01, 2017
p42 MAP Kinase (Mitogen-Activated Protein Kinase, MAPK), also known as Erk2 (Extracellular signal-regulated kinase 2) is one of two isoforms of MAP kinase family. It is a serine/threonine protein kinase, participating in mammalian signal transduction pathways that control intracellular responses to hormones and major developmental changes. Full activation of p42 MAP Kinase requires phosphorylation at residues T183 and Y185 catalyzed by the upstream protein kinase MEK2 or MAP Kinase Kinase (MAPKK) (1-4).
- Protein serine/threonine kinase
- Mouse, recombinant (E. coli)
Product SourceIsolated from a strain of E. coli that carries a clone expressing murine MAPK (1) under the control of a T7 expression system. Fully active MAPK is produced by co-expression with a constitutively active form of its activator, MEK2 (6).
Recognition DeterminantThe minimal recognition motif for phosphorylation by MAPK is S/TP. Pro is also common at the -2 position in the optimal primary motif PXS/TP. The substrate specificity of MAPK overlaps with other proline-directed protein kinases present within the cell. This suggests that the recognition of protein substrates may require structural determinants in addition to primary sequence requirements (5).
The following reagents are supplied with this product:
Store at (°C) Concentration NEBuffer™ for Protein Kinases (PK) -20 10 X
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