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Calmodulin-dependent Protein Kinase II (CaMKII)

  • This product was discontinued on January 01, 2017
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Ordering Information

P6060
  • Discontinued
    Discontinued
  • Product Information
    Calmodulin-Dependent Protein Kinase II (CaMKII) is a serine/threonine kinase. It is a Ca2+/calmodulin-dependent, truncated monomer (1-325 amino acid residues) of the α subunit. Autophosphorylation of threonine 286 in the presence of Ca2+ and calmodulin activates CaMKII and produces substantial Ca2+/calmodulin-independent activity (1,2). 

    Highlights

    • Protein serine/threonine kinase
    • Rat, recombinant (S. frugiperda Sf9)

    Product Source

    Isolated from Spodoptera frugiperda (Sf9) cells infected with recombinant baculovirus carrying the rat truncated CaMKII (kindly provided by Dr. H. Shulman).

    Recognition Determinant

    The minimal recognition motif for phosphorylation by CaMKII is RXXS/T. A more recent report suggests the presence of positive determinants at the -5, -2 and +1 positions in addition to the -3R. Thus, CaMKII preferentially phosphorylates substrates with motifs: HydXRXXS/T and HydXRNBXS/T, respectively, where Hyd represents a hydrophobic, X any, and NB a non-basic amino acid residue (3).

    Reagents Supplied

    The following reagents are supplied with this product:

    Store at (°C) Concentration
    Adenosine-5'-Triphosphate (ATP) -20 10 mM
    NEBuffer™ for Protein Kinases (PK) -20 10 X
    CaMKII Reaction Buffer Pack 12 μM
    CaCl2 (10X) 20 mM
    Product Categories:
    Protein Kinases Information
    Applications:
    Protein Phosphatases & Kinases
    • Properties and Usage
    • Related Products
    • Notes
    • References
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