Calmodulin-dependent Protein Kinase II (CaMKII)
- This product was discontinued on January 01, 2017
Calmodulin-Dependent Protein Kinase II (CaMKII) is a serine/threonine kinase. It is a Ca2+/calmodulin-dependent, truncated monomer (1-325 amino acid residues) of the α subunit. Autophosphorylation of threonine 286 in the presence of Ca2+ and calmodulin activates CaMKII and produces substantial Ca2+/calmodulin-independent activity (1,2).
- Protein serine/threonine kinase
- Rat, recombinant (S. frugiperda Sf9)
Product SourceIsolated from Spodoptera frugiperda (Sf9) cells infected with recombinant baculovirus carrying the rat truncated CaMKII (kindly provided by Dr. H. Shulman).
Recognition DeterminantThe minimal recognition motif for phosphorylation by CaMKII is RXXS/T. A more recent report suggests the presence of positive determinants at the -5, -2 and +1 positions in addition to the -3R. Thus, CaMKII preferentially phosphorylates substrates with motifs: HydXRXXS/T and HydXRNBXS/T, respectively, where Hyd represents a hydrophobic, X any, and NB a non-basic amino acid residue (3).
The following reagents are supplied with this product:
Store at (°C) Concentration Adenosine-5'-Triphosphate (ATP) -20 10 mM NEBuffer™ for Protein Kinases (PK) -20 10 X CaMKII Reaction Buffer Pack 12 μM CaCl2 (10X) 20 mM
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