Glycogen Synthase Kinase 3 (GSK-3)

  • This product was discontinued on January 01, 2017
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Ordering Information

  • Discontinued
  • Product Information
    Glycogen Synthase Kinase 3 (GSK­‑3) is a serine/threonine protein kinase and one of several protein kinases, which phosphorylate glycogen synthase. It is also called Factor A (FA) for its ability to activate the MgATP-dependent form of the protein phosphatase PP1 called FC (1-4). Recent studies demonstrate that GSK-3 can autophosphorylate Ser, Thr and Tyr. Ser/Thr phosphorylation causes inactivation, and Tyr phosphorylation results in increased activity (Y216 for GSK-3β). GSK-3 expressed in E. coli or insect cells is extensively phosphorylated on Tyr. Molecules lacking phosphate at this position can autophosphorylate after incubation with Mg2+ and ATP. GSK-3 phosphorylates several exogenous substrates, but not on Tyr residues (5,6). 

    • Protein serine/threonine kinase
    • Rabbit skeletal muscle, recombinant (E. coli)
    • Supplied with 10X Reaction Buffer

    Product Source

    Isolated from a strain of E. coli that carries a clone expressing GSK-3β derived from a rabbit skeletal muscle cDNA library (kindly provided by Dr. P. J. Roach) (5).

    Recognition Determinant

    The substrate specificity of GSK-3 is unique and substrate dependent. For some substrates, prior phosphorylation of the substrate to form the motif S/TXXXpS/pT is a strict requirement whereas in other substrates, no previous phosphorylation is needed. In either case, many of the GSK-3 sites have Pro residues close to the modified Ser or Thr (5,7).

    Reagents Supplied

    The following reagents are supplied with this product:

    Store at (°C) Concentration
    NEBuffer™ for Protein Kinases (PK) -20 10 X
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