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  • Sodium Fluoride (Fluoride)

    Description

    Sodium Fluoride (Fluoride, NaF) is a commonly used general inhibitor for protein phosphoseryl and phosphothreonyl phosphatases (PSPs). Routinely Fluoride, combined with Sodium Orthovanadate (NEB# P0758), a general inhibitor for protein phosphotyrosyl phosphatases (PTPs), is used to preserve the protein phosphorylation state in cells, cell lysates, and protein kinase (PK) assays. These inhibitors should ideally be present in all buffers used in the purification of PKs (1, 2). 

    Properties and Usage

    Storage Temperature

    -20°C

    References

    1. Gordon, J.A. (1991). Methods in Enzymology. 201, 477-482.
    2. Hardie, D.G. et al. (1993). Protein Phosphorylation: A Practical Approach. NY: IRL Press. 62.

    Supporting Documents

    Material Safety Datasheets

    The following is a list of Material Safety Data Sheets (MSDS) that apply to this product to help you use it safely. The following file naming structure is used to name these document files: [Product Name] MSDS. For international versions please contact us at info@neb.com.

    Datacards

    The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.