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PCR & Reaction Cleanup

The Monarch PCR & DNA Cleanup Kit reliably purifies up to 5 µg of concentrated, high quality DNA from PCR and other enzymatic reactions. It utilizes a bind/wash/elute workflow with minimal incubation and spin times. The columns provided with each kit ensure zero buffer retention and no carryover of contaminants, enabling elution in volumes as low as 6 µl. Monarch Buffers have been optimized, and do not require pH monitoring. Eluted DNA is ready for use in restriction digests, DNA sequencing, PCR, ligation, and other enzymatic manipulations.


Monarch PCR & DNA Cleanup Kits provide fast and reliable purification, reduced hands-on time and elution in low volumes

Our unique column design offers several improvements over other commercially available products, including the elimination of buffer retention and elution in as little as 6 µl

FAQs for PCR & Reaction Cleanup

Protocols for PCR & Reaction Cleanup

Product Manual

Advantages of the Monarch PCR & DNA Cleanup Kit

  • Elute in as little as 6 μl
  • Prevent buffer retention and salt carryover with optimized column design
  • Save time with fast, user-friendly protocols
  • No need to monitor pH
  • Buffers and columns available separately
  • Significantly less plastic used when compared with other kits
  • Responsibly-sourced and recyclable packaging

Specifications of the Monarch PCR & DNA Cleanup Kit

  • Binding Capacity: up to 5 μg
  • DNA Size Range:
    ~50 bp to 25 kb
    DNA ≥  15 bp to 25 kb (dsDNA) and DNA ≥ 18 nt to 10 kb (ssDNA) can also be purified using the Oligonucleotide Cleanup Protocol.
  • Typical Recovery:
    DNA (50 bp to 10 kb): 70–90%
    DNA (11–25 kb): 50–70%
    ssDNA ≥  18 nt and dsDNA ≥ 15 bp: 70-85%
  • Elution Volume: ≥ 6 μl
  • Purity: A260/280 ≥ 1.8
  • Protocol Time: 5 min of spin and incubation time
  • Compatible Downstream Applications: ligation, restriction digestion, labeling and other enzymatic manipulations, library construction and DNA sequencing

Legal Information

This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).

While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.

For more information about commercial rights, please contact NEB's Global Business Development team at

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

  1. Monarch_PCRCleanupProtocol_VideoThumb

    Monarch PCR & DNA Cleanup Kit protocol

    Learn how to isolate DNA from your enzymatic reactions, including PCR, using the Monarch PCR & DNA Cleanup Kit (5 µg).

  2. monarch_pcrcleanuptips_videothumb

    Tips for using the Monarch PCR & DNA Cleanup Kit

    Optimize your DNA isolation from PCR and other enzymatic reactions with our quick tips for using the Monarch PCR & DNA Cleanup Kit.

  3. Monarch_HowToRecycle_VideoThumb

    How to recycle your Monarch Kit components

    Learn how you can easily recycle all of the components in your Monarch Kits.