DNA Cleanup
Learn more about Monarch Kits, and request a free sample at NEBMonarch.com
Our unique column design offers several improvements over other commercially available products, including the elimination of buffer retention and elution in as little as 6 µl
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FAQs for DNA Cleanup
- Can I use water to elute the DNA when using the Monarch Kits?
- What is the smallest volume of elution buffer that can be used with the Monarch DNA Cleanup Column?
- After purification, I see a faint additional band running below the expected size on a gel. What happened?
- Are Monarch spin columns compatible with Vacuum Manifolds?
- Are the columns in the Monarch PCR & DNA Cleanup Kit (5 μg) the same as the ones in the Monarch DNA Gel Extraction Kit?
- Can the Monarch PCR & DNA Cleanup Kit (5 μg) be used to purify RNA?
- Do you have any recommendations for purification of ssDNA?
- What factors affect my (A260/A230)?
- What is the composition of each buffer provided with the Monarch PCR & DNA Cleanup Kit (5 μg)?
- What is the maximum binding capacity of the Monarch DNA Cleanup Column provided with the Monarch PCR & DNA Cleanup Kit (5 μg)?
- What size of DNA can be purified with the Monarch DNA Cleanup Columns?
- What size primers can be effectively removed from a PCR reaction?
- Can I use the Monarch DNA & PCR Cleanup Kit to purify oligonucleotides and other short DNA fragments?
Protocols for DNA Cleanup
Application Notes for DNA Cleanup
Product Manual
Advantages of the Monarch PCR & DNA Cleanup Kit
- Elute in as little as 6 μl
- Prevent buffer retention and salt carryover with optimized column design
- Save time with fast, user-friendly protocols
- No need to monitor pH
- Buffers and columns available separately
- Significantly less plastic used when compared with other kits
- Responsibly-sourced and recyclable packaging
Specifications of the Monarch PCR & DNA Cleanup Kit
- Binding Capacity: up to 5 μg
- DNA Size Range:
~50 bp to 25 kb
DNA ≥ 15 bp to 25 kb (dsDNA) and DNA ≥ 18 nt to 10 kb (ssDNA) can also be purified using the Oligonucleotide Cleanup Protocol. - Typical Recovery:
DNA (50 bp to 10 kb): 70–90%
DNA (11–25 kb): 50–70%
ssDNA ≥ 18 nt and dsDNA ≥ 15 bp: 70-85% - Elution Volume: ≥ 6 μl
- Purity: A260/280 ≥ 1.8
- Protocol Time: 5 min of spin and incubation time
- Compatible Downstream Applications: ligation, restriction digestion, labeling and other enzymatic manipulations, library construction and DNA sequencing
Legal Information
This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).
While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.
For more information about commercial rights, please contact NEB's Global Business Development team at gbd@neb.com.
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
