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NEBNext Direct® offers a novel approach to NGS Target Enrichment. Using a unique, capture-based protocol, NEBNext Direct offers a range of solutions for highly specific, efficient targeting of genomic loci of interest. Optimized, 1-day protocols allow for rapid profiling of targeted regions for a variety of applications, including germline and somatic variant calling for translational research and genotyping of genomic markers for applications in agricultural biology and sample identification.

Define your own targeted sequencing panel by selecting only the genes you want from our expansive list of genes using NEBNext Direct Custom Ready Panels. For genotyping applications, the NEBNext Genotyping Solution enables pre-capture pooling of up to 96 samples for cost-effective and scalable genotyping of up to 5,000 genomic markers.

  1. Highly multiplexed capture of genomic markers for high throughput NGS based genotyping

    Highly multiplexed capture of genomic markers for high throughput NGS based genotyping

    In this ESHG 2020 workshop, hear about methylation analysis of cfDNA samples, sample identification and tracking using a high-throughput SNP genotyping method and the importance of sample prep in large-scale sequencing pipelines.

  2. Fully-Automated-Targeted-SNP-Genotyping-for-Plant-and-Animal-Applications

    Fully Automated Targeted SNP Genotyping for Plant and Animal Applications

    This webinar from NEB®, EMBL and Beckman Coulter® Life Sciences discusses the development and verification of the automated NEBNext Direct® Genotyping Solution on the Beckman Coulter i7 workstation to achieve highly-multiplexed sample processing and meet the growing demands of plant and animal genotyping applications.

  3. NEB TV

    NEB® TV Ep. 11 – Target Enrichment for NGS

    Precision medicine requires precision enrichment. Learn more about target enrichment and its role in clinical applications.

NEBNext Direct Workflow

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Advantages

  • Choose from a single gene to hundreds of genes

  • Experience unmatched specificity and coverage uniformity

  • Eliminate synthesis and optimization steps for faster turnaround

  • Improve sensitivity with our Unique Molecular Identifier (UMI)

  • Generate results in one day with our automation-friendly workflow  

NEW ENGLAND BIOLABS® and NEB® are registered trademarks of New England Biolabs, Inc.
NEBNext Direct® is a trademark of New England Biolabs, Inc.
Directed Genomics™ is a trademark of Directed Genomics