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RNA Library Prep

NEBNext® Ultra™ II kits are available for directional (strand-specific) or non-directional RNA library preparation. These kits utilize streamlined workflows and novel master mixes, and have been designed for performance with input amounts as low as 5 ng Total RNA.

Modules offer the user ability to customize sample preparation, and adaptors and primers (NEBNext Oligos) are available separately, for maximum flexibility.

  • The NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (NEB #E7760) uses the dUTP method and a streamlined workflow to generate high yield, high quality strand-specific libraries.
  • The NEBNext® Ultra™ II RNA Library Prep Kit for Illumina® (NEB #E7770) enables production of non-directional libraries from as little as 10 ng of Total RNA, using a streamlined workflow.

Removal of abundant RNAs, such as ribosomal RNA, is enabled by a growing range of RNA Depletion kits, for human, mouse, rat, bacteria and blood samples, in addition to a kit for poly(A) mRNA enrichment. Customized RNA depletion from any organism is now an option, with a user-friendly depletion probe design tool and reagent set.

For microRNAs and other small RNAs, our novel Small RNA workflow has been optimized to minimize adaptor-dimers, while producing high-yield, high-density libraries.

For single cell and ultra-low input RNA, our simple and robust workflow generates full length cDNAs, followed by conversion to sequence-ready libraries using the Ultra II FS workflow, that incorporates enzymatic DNA fragmentation.

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FAQs for RNA Library Prep
Protocols for RNA Library Prep
Other Tools and Resources
Single Cell/Low Input RNA Library Preparation Workflow for Illumina
Ultra II Directional RNA Library Preparation Workflow for Illumina
Ultra II  Non-directional RNA Library Preparation Workflow for Illumina
NEBNext rRNA Depletion Kit Workflow
Small RNA Library Preparation Workflow for Illumina
Legal Information

This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).

While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.

For more information about commercial rights, please email us at

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.