NEBNext® Ultra™ II kits are available for directional (strand-specific) or non-directional RNA library preparation. These kits utilize streamlined workflows and novel master mixes, and have been designed for performance with input amounts as low as 5 ng Total RNA.
Modules offer the user ability to customize sample preparation, and adaptors and primers (NEBNext Oligos) are available separately, for maximum flexibility.
- The NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (NEB #E7760) uses the dUTP method and a streamlined workflow to generate high yield, high quality strand-specific libraries.
- The NEBNext® Ultra™ II RNA Library Prep Kit for Illumina® (NEB #E7770) enables production of non-directional libraries from as little as 10 ng of Total RNA, using a streamlined workflow.
Removal of abundant RNAs, such as ribosomal RNA, is enabled by a growing range of RNA Depletion kits, for human, mouse, rat, bacteria and blood samples, in addition to a kit for poly(A) mRNA enrichment. Customized RNA depletion from any organism is now an option, with a user-friendly depletion probe design tool and reagent set.
For microRNAs and other small RNAs, our novel Small RNA workflow has been optimized to minimize adaptor-dimers, while producing high-yield, high-density libraries.
For single cell and ultra-low input RNA, our simple and robust workflow generates full length cDNAs, followed by conversion to sequence-ready libraries using the Ultra™ II FS workflow, that incorporates enzymatic DNA fragmentation.
Watch as Deyra explains how to optimize your RNA inputs for successful NGS library preparation.
The common problem of adaptor dimer formation during small RNA library construction can be avoided by using NEBNext® protocols. Learn about this technique, and how it improves both performance and sensitivity in library construction.
Using beads to clean up your DNA prior to NGS library prep can be quick and easy, if you follow these few, simple tips.
These 12 quick tips for NGS library preparation are a great refresher if you're a seasoned pro, or a great introduction if you're a beginner.