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Adaptors and Primers for Library Prep

Adaptors and Primers are an essential component of your NGS sample prep. NEBNext Oligos offer flexibility in multiplexing with a wide range of highly pure index primers, available in unique dual, dual, and single index-primer formats. NEBNext adaptors are designed to optimize yield while minimizing adaptor-dimer formation, and the NEBNext Adaptor, with its unique hairpin-loop structure, offers high ligation efficiency.  

The EM-seq™ Adaptor was optimized for use with NEBNext’s Enzymatic Methyl-seq protocol, a gentler alternative to the harsh sample processing in bisulfite sequencing, and is also compatible with bisulfite sequencing workflows.

NEBNext Oligos are supplied separately from NEBNext Library Prep Kits (except for the Small RNA and EM-seq Kits) to provide workflow flexibility.

NEBNext Oligos currently enable multiplexing of up to 384 unique dual indexed reactions, up to 384 dual indexed reactions (using combinatorial indexing), and up to 144 single indexed reactions. For additional information, refer to the NEBNext Multiplex Oligos Selection Chart.   

 
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FAQs for Adaptors and Primers for Library Prep
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Workflow demonstrating the use of the NEBNext Adaptor and index primers
Benefits

Benefits of NEBNext Adaptors and Primers:

  • Increased ligation efficiency 
  • Minimized adaptor-dimer formation 
  • Increased library yields 
  • Dual barcodes for increased sample identification specificity
    • Unique dual index pairs enable barcode hopping detection (384 pairs available)
    • 2 sets of non-unique/combinatorial dual index primers available 
  • Large number of single barcodes available
    • 4 sets of 12 indices
    • 1 set of 96 indices supplied in a 96-well plate with a pierceable foil seal 
  • Index pooling guidelines and sample sheets are provided 
Legal Information

This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).

While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.

For more information about commercial rights, please contact NEB's Global Business Development team at [email protected].

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.


Videos

  1. NEBNext_UltraII_Protocol_282x210

    NEBNext Ultra II DNA Library Prep Protocol

    This video walks you through DNA Library Preparation using the NEBNext Ultra II DNA Library Prep Kit. The video also includes tips for optimization as well as safe stopping points.

  2. NEBNextUltra2DirectionalRNA_720

    NEBNext Ultra II Directional RNA Workflow

    Learn more about the streamlined workflow for the NEBNext Ultra II Directional RNA Library Prep Kit.

  3. EMseqWorkflow_Animation_Thumb_720

    NEBNext® Enzymatic Methyl-seq Workflow

    A workflow animation for an enzyme-based method for detection of 5mC and 5hmC, an alternative to bisulfite sequencing.

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