For more information about the importance of quantitation, read “The Quantitation Question: How does accurate library quantitation influence sequencing?”.
The NEBNext® Library Quant Kit for Illumina® is an optimized set of pre-diluted DNA standards and enzyme master mix for improved qPCR-based library quant for Illumina sequencing. Now with six standards for added flexibility, you can choose the right number of standards for your application. ROX is included in the kit for use with qPCR instruments that require a reference dye for normalization. Functionally validated to meet stringent criteria set for assay efficiency and quantitation cycles (Cq) for each DNA standard and no-template control reaction. Additional volume of the six DNA standards and dilution buffer are available separately.
The Quantitation Question: How does accurate library quantitation influence sequencing?
The determination of the number of sequencing-ready molecules present after library preparation is an important step in the next generation sequencing (NGS) workflow and has a strong influence on the success of both a sequencing run and a sequencing-based experiment.
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