Library Preparation for Oxford Nanopore Technologies

Oxford Nanopore Technology (ONT) Sequencing methodology for next generation sequencing (NGS) wherein a motor protein passes a nucleic acid molecule through a small hole, or “nanopore”, in a flow cell, detecting the change in voltage as each base passes. This rethinking of NGS by Oxford Nanopore Technology has enabled the realization of smaller, more portable, and less expensive NGS platforms with applications in robust field-based studies as well as in fully outfitted laboratories.  
In addition to individual reagents included in protocols from Oxford Nanopore Technologies for multiple applications, NEBNext® also provides companion reagent products tailored for use in specific workflows, in conjunction with Oxford Nanopore Technology’s own library prep and sequencing reagents: 

  • NEBNext® Companion Module for Oxford Nanopore Technologies® Ligation Sequencing (NEB #E7180
  • NEBNext® ARTIC SARS-CoV-2 Companion Kit (Oxford Nanopore Technologies®) (NEB #E7660

For more information about the NEBNext ARTIC kit and workflow, view our Infectious Disease & ARTIC product page


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Protocols for Library Preparation for Oxford Nanopore Technologies
NEBNext ARTIC SARS-CoV-2 Workflow for Oxford Nanopore Technologies Sequencing
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