Illumina® sequencing instruments can be found in laboratories of all sizes, from lab-scale to production-scale, and NEBNext kits can be used to construct the high-quality libraries required for them all, for a wide array of sample types and applications.
NGS Library Preperation
The NEBNext® suite of products supports Illumina sequencing with sample preparation tools that streamline workflows, minimize inputs, and improve library yields and quality. NEBNext library preparation kits are available for both DNA library prep and RNA library prep; kits and modules are available for genomic DNA, FFPE DNA, methylated DNA, microbiome DNA, total RNA, single cell/low input RNA, and small RNA samples. These are complemented by a wide range of index primers and index adaptors for multiplexing. In addition to the extensive QCs performed on individual kit components, all NEBNext kits for Illumina library prep are functionally validated by preparation of a library, followed by Illumina sequencing. NEBNext library preparation reagents are available in multiple kit and workflow formats, for maximum convenience and flexibility. Designed for use in concert with NEBNext Multiplex Oligos for simple yet powerful NGS sample multiplexing.
Introducing: NEBNext UltraExpress™ library prep
Streamlined for speed.
The NEBNext UltraExpress library prep kits are the latest generation of library prep reagents for DNA or RNA, designed to be faster, simpler, and even more automation friendly. The new kits generate high-quality libraries from a variety of sample types with a single protocol for all inputs within the specified input range. The workflows incorporate master mixed reagents, reduced incubation times, and fewer cleanup steps, keeping hands-on time at a minimum.
- NEBNext UltraExpress DNA Library Prep
NEBNext Ultra™ II is at the heart of our DNA and RNA library prep workflows.
The NEBNext Ultra II reagents and workflow are at the heart of NEB’s portfolio for next generation sequencing library preparation. NEBNext Ultra II kits and modules for Illumina® are an optimized combination of reagents and simplified workflows, enabling you to create DNA or RNA libraries of highest quality and yield, even when starting from extremely low or low-quality input amounts.
The Ultra II workflow is at the heart of:
- Ultra II DNA & FS DNA Library Prep, with PCR-free workflow options
- Enzymatic Methyl-seq
- Ultra II RNA & Directional RNA Library Prep
- Single Cell/Low Input RNA Library Prep
- NEBNext ARTIC SARS-CoV-2 library prep
- Modular options for each step in the workflow
Don’t just take our word for it; review the nearly 25,000 publications featuring NEBNext reagents, instead.
Improving Enzymatic DNA Fragmentation for Next Generation Sequencing Library Construction
Among the available options for DNA fragmentation, enzymatic fragmentation is demonstrably gentler on the sample, yielding less damage at any scale. The new NEBNext® Ultra™ II FS DNA Library Prep Kit for Illumina® includes a single step for enzymatic fragmentation, end repair, and dA tailing.
- NEBNext Selector
- A Robust, Streamlined, Enzyme-based DNA Library Preparation Method Amenable to a Wide Range of DNA Inputs (2019)
- EM-seq™ Enables Accurate and Precise Methylome Analysis of Challenging DNA Samples (2019)
- Improving Transcriptome Profiling for Single Cell and Low Input RNA (2019)
- NEBNext Direct® Custom Ready Panels Overcome Challenges Associated with Targeted Re-sequencing (2019)
- NEBNext® Ultra™II FS DNA: A Robust Enzyme-based DNA Library Preparation Method Compatible with Plant and Animal Samples (2019)
- Uncovering the Cannabis sativa Methylome Through Enzymatic Methyl-seq (2019)
Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email email@example.com.
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
This video demonstrates the "Phased Cleanup" step for NEBNext UltraExpress™ Kits, a new method that improves library separation from smaller fragments, including adaptor dimer.
Learn more about the streamlined workflow for the NEBNext Ultra II Directional RNA Library Prep Kit.
This video walks you through the size selection and cleanup steps using the NEBNext Ultra II DNA
Optimizing DNA inputs for NGS library prep is an important step, if you want to ensure a high-quality library. Start by following these tips for DNA quantification and assessing purity.
Watch as Deyra explains how to optimize your RNA inputs for successful NGS library preparation.
This video walks you through DNA Library Preparation using the NEBNext Ultra II DNA Library Prep Kit. The video also includes tips for optimization as well as safe stopping points.
These 12 quick tips for NGS library preparation are a great refresher if you're a seasoned pro, or a great introduction if you're a beginner.
Are you running a core sequencing facility? Watch as Eileen shares a few reasons that NEBNext products are particularly well suited to sequencing core labs.
Behind the paper: DNA damage is a pervasive cause of sequencing errors, directly confounding variant identification
NEB researchers published a paper in Science highlighting DNA damage as a prevalent source of errors in public cancer databases. Learn about how addressing this damage could improve the detection of low-frequency disease variants.
In this webinar, Laurence Etwiller and Jennifer Ong discuss their recent publications, and how library preparation affects sequencing accuracy and variant calling. View full Q & A summary.