Library Preparation for Illumina

Illumina^® sequencing instruments can be found in laboratories of all sizes, from lab-scale to production-scale, and NEBNext kits can be used to construct the high-quality libraries required for them all, for a wide array of sample types and applications.

NGS Library Preperation

The NEBNext^® suite of products supports Illumina sequencing with sample preparation tools that streamline workflows, minimize inputs, and improve library yields and quality. NEBNext library preparation kits are available for both DNA library prep and RNA library prep; kits and modules are available for genomic DNA, FFPE DNA, methylated DNA, microbiome DNA, total RNA, single cell/low input RNA, and small RNA samples. These are complemented by a wide range of index primers and index adaptors for multiplexing. In addition to the extensive QCs performed on individual kit components, all NEBNext kits for Illumina library prep are functionally validated by preparation of a library, followed by Illumina sequencing. NEBNext library preparation reagents are available in multiple kit and workflow formats, for maximum convenience and flexibility. Designed for use in concert with NEBNext Multiplex Oligos for simple yet powerful NGS sample multiplexing.

Introducing: NEBNext UltraExpress^® library prep
Streamlined for speed.

The NEBNext UltraExpress library prep kits are the latest generation of library prep reagents for DNA or RNA, designed to be faster, simpler, and even more automation friendly. The new kits generate high-quality libraries from a variety of sample types with a single protocol for all inputs within the specified input range. The workflows incorporate master mixed reagents, reduced incubation times, and fewer cleanup steps, keeping hands-on time at a minimum.

NEBNext UltraExpress DNA Library Prep Workflow

NEBNext UltraExpress FS DNA Library Prep Kit Workflow

NEBNext UltraExpress RNA Library Prep Kit Workflow

Savings* with NEBNext UltraExpress

* As compared to NEBNext Ultra II

NEBNext Ultra™ II is at the heart of our DNA and RNA library prep workflows.

The NEBNext Ultra II reagents and workflow are at the heart of NEB’s portfolio for next generation sequencing library preparation. NEBNext Ultra II kits and modules for Illumina® are an optimized combination of reagents and simplified workflows, enabling you to create DNA or RNA libraries of highest quality and yield, even when starting from extremely low or low-quality input amounts.

The Ultra II workflow is at the heart of: 

• Ultra II DNA & FS DNA Library Prep, with PCR-free workflow options
• Enzymatic Methyl-seq
• Ultra II RNA & Directional RNA Library Prep
• Single Cell/Low Input RNA Library Prep  
• NEBNext ARTIC SARS-CoV-2 library prep 
• Modular options for each step in the workflow
  
  

NEBNext Ultra II DNA Library Prep Workflow

Don’t just take our word for it; review the nearly 25,000 publications featuring NEBNext reagents, instead.