There are two steps to using this system: subcloning and expression of the protein of interest as an ACP-tag fusion, and labeling of the fusion protein with the CoA substrate of choice. The cloning and expression of ACP-tag protein fusions is described in documents provided with the pACP-tag(m)-2 cloning plasmid. The labeling of fusion proteins with CoA substrates is described in the documentation supplied with CoA substrates and ACP or SFP Synthase.
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