pACP-ADRβ2 Control Plasmid
- This product was discontinued on January 01, 2013
This control plasmid contains the gene encoding the Beta-2 Adrenergic Receptor cloned as a fusion to the C-terminus of the ACP-tag. The signal peptide fused to the N-terminus of ACP-tag is based on the 5HT3A Serotonin Receptor. The Beta-2 Adrenergic Receptor is a member of the G protein coupled receptors and mediates the catecholamine-induced activation of adenylate cyclase through the action of G proteins.
The ACP-Beta-2 Adrenergic Receptor is inserted in the plasma membrane with the ACP-tag exposed to the extracellular side of the membrane. When labeled with CoA substrates, it gives a selective cell membrane fluorescence labeling pattern.The full sequence and map for pACP-ADRβ2 can be downloaded here.
The ACP-tag is a small protein tag (8 kDa) based on the acyl carrier protein (ACP). It allows the specific, covalent attachment of virtually any molecule to a protein of interest. ACP-tag substrates are derivatives of coenzyme A (CoA). In the labeling reaction, the substituted phosphopantetheine group of CoA is covalently attached to a conserved serine residue of the ACP-tag by a phosphopantetheine transferase (ACP or SFP Synthase). Having no cysteines, the ACP-tag is particularly suited for specifically labeling cell-surface proteins, and should be useful for labeling secreted proteins with disulfide bridges such as antibodies.
There are two steps to using this system: subcloning and expression of the protein of interest as an ACP-tag fusion, and labeling of the fusion protein with the CoA substrate of choice. The cloning and expression of ACP-tag protein fusions is described in documents provided with the pACP-tag(m)-2 cloning plasmid. The labeling of fusion proteins with CoA substrates is described in the documentation supplied with CoA substrates and ACP or SFP Synthase.
- Product Categories:
- Discontinued Products
- FAQs & Tech Tips
- Protocols & Manuals
- Other Tools & Resources
- Quality & Safety
- Legal Information
Ineligible item added to cart
Based on your Freezer Program type, you are trying to add a product to your cart that is either not allowed or not allowed with the existing contents of your cart. Please review and update your order accordingly If you have any questions, please contact Customer Service at firstname.lastname@example.org or 1-800-632-5227 x 8.