There are two steps to using this system: sub cloning and expression of the protein of interest as an MCP-tag fusion, and labeling of the fusion protein using SFP Synthase with the substrate of choice.The cloning and expression of MCP-tag protein fusions is described in documents provided with the pMCP-tag(m) cloning plasmid. The labeling of fusion proteins with CoA substrates is described in the documentation supplied with CoA substrates and SFP Synthase.
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