pSNAP-tag® (T7)-2 Vector

pSNAP-tag® (T7)-2 Vector is an Escherichia coli expression plasmid encoding the SNAP-tag protein. Expression is under control of the IPTG inducible T7 promoter.

  • This plasmid is intended as an expression vector for SNAP-tag gene fusions in appropriate E. coli strains
  • The E. coli strain used for expression needs to provide T7 polymerase in order to achieve expression from the T7 promoter upstream of the gene
  • The vector can be used for expression of the SNAP-tag protein alone (20 kDa)
  • Gene of interest can be fused to the N-terminus or C-terminus of SNAP-tag 

Ordering Information

N9181S_pdp_800x450
  • 20 μg
    $157.00
  • Product Information
    pSNAP-tag (T7)-2 Vector is an Escherichia coli expression plasmid encoding the SNAP-tag protein. Expression is under control of the IPTG inducible T7 promoter. The pSNAP-tag (T7)-2 Vector can also be used as a control plasmid expressing the SNAP-tag protein (20 kDa). The target gene should be cloned as a fusion to the N- or C-terminus of the SNAP-tag.

    The SNAP-tag is a novel tool for protein research, allowing the specific, covalent attachment of virtually any molecule to a protein of interest. The SNAP-tag is a protein based on human O6-alkylguanine-DNA-alkyltransferase (hAGT)(1,2,3). SNAP-tag substrates are derivatives of benzyl purines and benzyl chloropyrimidines. In the labeling reaction, the substituted benzyl group of the substrate is covalently attached to the SNAP-tag.

    There are two steps to using this system: sub cloning and expression of the protein of interest as a SNAP-tag fusion, and labeling of the fusion with the SNAP-tag substrate of choice. Cloning and expression of SNAP-tag fusion proteins are described in this document. The labeling of the fusion proteins with SNAP-tag substrates is described in the instructions supplied with SNAP-tag substrates. 
    Cloning Region of pSNAP-tag® (T7)-2 Vector Cloning Region of pSNAP-tag® (T7)-2 Vector



    Unique restriction sites in the regions flanking the SNAP26b gene are displayed above the coding strand.

    Product Categories:
    Cellular Analysis Vectors,
    DNA Plasmids & Substrates,
    Protein Labeling
    Applications:
    SNAP Surface,
    In vivo Imaging,
    Pulse Chase
    ,
    Receptor Internalization
    ,
    Protein Localization
    ,
    e-coli-protein-expression
    ,
    protein-labeling-snap-clip
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