- Catalog # N8084 was discontinued on January 01, 2017
The pTK-GLuc Vector is a mammalian expression vector that encodes the secreted luciferase from the copepod Gaussia princeps as a reporter, under the control of the constitutive Herpes Simplex Virus thymidine kinase promoter. Gaussia Luciferase (GLuc) is a 20 kDa protein encoded by a "humanized" sequence, and it contains a native signal peptide at the N-terminus that allows it to be secreted from mammalian cells into the cell culture medium (1,2). pTK-GLuc has a multiple cloning site (MCS) between the GLuc stop codon and the polyadenylation site. A neomycin resistance gene under the control of an SV40 promoter allows selection for stable integration of the plasmid into the mammalian cell genome using G418.
- TK promoter: 18–771
- GLuc coding: 802–1359
- Start codon: 802–804
- Stop codon: 1357–1359
- Signal peptide: 802–852
- Multiple cloning site (MCS) downstream of GLuc: 1360–1391 (NotI, AgeI, XhoI, XbaI)
- SV40 late polyadenylation signal: 1392–1622
- Neo promoter (SV40): 2215–2550
- Neomycin resistance gene 2602–3396
- NeoR poly-A(SV40 early): 3570–3700
- Bacterial replication ori (pMB1) 4730–4142
- Amp resistance 5761–4901
Product SourceIsolated from E. coli strain NEB10β by a standard DNA purification procedure.
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