pTK-GLuc Vector

This product has been discontinued and replaced by N8082.
  • Catalog # N8084 was discontinued on January 01, 2017 ;
Learn more about how this product is being used in the Product Citation Tool
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  • Product Information
    The pTK-GLuc Vector is a mammalian expression vector that encodes the secreted luciferase from the copepod Gaussia princeps as a reporter, under the control of the constitutive Herpes Simplex Virus thymidine kinase promoter. Gaussia Luciferase (GLuc) is a 20 kDa protein encoded by a "humanized" sequence, and it contains a native signal peptide at the N-terminus that allows it to be secreted from mammalian cells into the cell culture medium (1,2). pTK-GLuc has a multiple cloning site (MCS) between the GLuc stop codon and the polyadenylation site. A neomycin resistance gene under the control of an SV40 promoter allows selection for stable integration of the plasmid into the mammalian cell genome using G418.
    Figure 1:  pTK-GLuc multiple cloning site (MCS) Figure 1:  pTK-GLuc multiple cloning site (MCS)
    N8084
    The Gaussia Luciferase sequence is shown with a blue background. Only unique restriction sites are shown.

    DNASU and Addgene are central repositories for plasmid clones and collections that may also be helpful.

    Highlights

    • TK promoter: 18–771
    • GLuc coding: 802–1359
    • Start codon: 802–804
    • Stop codon: 1357–1359
    • Signal peptide: 802–852
    • Multiple cloning site (MCS) downstream of GLuc: 1360–1391 (NotI, AgeI, XhoI, XbaI)
    • SV40 late polyadenylation signal: 1392–1622
    • Neo promoter (SV40): 2215–2550
    • Neomycin resistance gene 2602–3396
    • NeoR poly-A(SV40 early): 3570–3700
    • Bacterial replication ori (pMB1) 4730–4142
    • Amp resistance 5761–4901

    Product Source

    Isolated from E. coli strain NEB10β by a standard DNA purification procedure.
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