The pTK-GLuc Vector is a mammalian expression vector that encodes the secreted luciferase from the copepod Gaussia princeps as a reporter, under the control of the constitutive Herpes Simplex Virus thymidine kinase promoter. Gaussia Luciferase (GLuc) is a 20 kDa protein encoded by a "humanized" sequence, and it contains a native signal peptide at the N-terminus that allows it to be secreted from mammalian cells into the cell culture medium (1,2). pTK-GLuc has a multiple cloning site (MCS) between the GLuc stop codon and the polyadenylation site. A neomycin resistance gene under the control of an SV40 promoter allows selection for stable integration of the plasmid into the mammalian cell genome using G418.
- TK promoter: 18–771
- GLuc coding: 802–1359
- Start codon: 802–804
- Stop codon: 1357–1359
- Signal peptide: 802–852
- Multiple cloning site (MCS) downstream of GLuc: 1360–1391 (NotI, AgeI, XhoI, XbaI)
- SV40 late polyadenylation signal: 1392–1622
- Neo promoter (SV40): 2215–2550
- Neomycin resistance gene 2602–3396
- NeoR poly-A(SV40 early): 3570–3700
- Bacterial replication ori (pMB1) 4730–4142
- Amp resistance 5761–4901
Product SourceIsolated from E. coli strain NEB10β by a standard DNA purification procedure.
- Product Categories:
- Discontinued Products
- Protocols, Manuals & Usage
- Tools & Resources
- FAQs & Troubleshooting
- Citations & Technical Literature
- Quality, Safety & Legal
Ineligible item added to cart
Based on your Freezer Program type, you are trying to add a product to your cart that is either not allowed or not allowed with the existing contents of your cart. Please review and update your order accordingly If you have any questions, please contact Customer Service at [email protected] or 1-800-632-5227 x 8.