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pGLuc-Basic 2 Vector

The pGLuc-Basic 2 Vector is a promoter-less vector for expression of GLuc under the control of sequences cloned in the MCS.

  • GLuc is codon-optimized for mammalian expression
  • GLuc can be assayed in the culture media or cell lysate
  • Can be used to generate Neomycin-resistant stable cell lines

Ordering Information

$name
  • 500 μg/ml
    20 μg
    $304.00
  • Product Information
    pGLuc-Basic 2 is a cloning vector for expression in mammalian cells, containing a reporter gene but lacking promoter elements. The reporter gene is the secreted luciferase from the copepod Gaussia princeps. Gaussia Luciferase (GLuc) is a 19 kDa protein encoded by a "humanized" sequence, and it contains a native signal peptide at the N-terminus that allows it to be secreted from mammalian cells into the cell culture medium (1,2). The pGLuc-Basic 2 Vector contains a multiple cloning site (MCS) upstream of the GLuc coding sequence. A neomycin resistance gene under the control of an SV40 promoter allows selection for stable integration of the plasmid into the mammalian cell genome using G418.

    Figure 1: Figure 1
    Comparison of light output obtained from HEK293 cells transfected with either the promoterless pGLuc-Basic 2 Vector or the pCMV-GLuc 2 Control Plasmid (NEB #N8081). Supernatants were harvested at 24 hour post-transfection and assayed for the GLuc activity using the BioLux GLuc Assay System.

    Figure 2: pGLuc-Basic multiple cloning site (MCS) Figure 2: pGLuc-Basic multiple cloning site (MCS)
    Figure 2: pGLuc-Basic multiple cloning site (MCS)
    The Gaussia Luciferase sequence is shown with a blue background. Only unique restriction sites are shown.

    DNASU and Addgene are central repositories for plasmid clones and collections that may also be helpful.

    Highlights

    • Polylinker MCS: 12–68
    • GLuc coding: 76–633
    • Start codon: 76–78
    • Stop codon: 631–633
    • Signal peptide: 76–126
    • Synthetic poly-A site: 642–690
    • Neo promoter (SV40): 1276–1611
    • Neomycin resistance gene: 1663–2457
    • Bacterial replication ori (pMB1): 3791–3203
    • Amp resistance: 4822–3962
    • All pGLuc 2 vectors and plasmids have improved polyadenylation-transcription termination of the luciferase transcript. The polyadenylation signal is a synthetic polyadenylation sequence based on the β-globin gene (5)

    Product Source

    Isolated from an E. coli strain NEB10b by standard DNA purification procedure.
    Product Categories:
    Gaussia Luciferase Products,
    DNA Plasmid & Substrates Products
    Applications:
    Gaussia Luciferase
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