pGLuc-Basic 2 Vector
The pGLuc-Basic 2 Vector is a promoter-less vector for expression of GLuc under the control of sequences cloned in the MCS.
- GLuc is codon-optimized for mammalian expression
- GLuc can be assayed in the culture media or cell lysate
- Can be used to generate Neomycin-resistant stable cell lines
pGLuc-Basic 2 is a cloning vector for expression in mammalian cells, containing a reporter gene but lacking promoter elements. The reporter gene is the secreted luciferase from the copepod Gaussia princeps. Gaussia Luciferase (GLuc) is a 19 kDa protein encoded by a "humanized" sequence, and it contains a native signal peptide at the N-terminus that allows it to be secreted from mammalian cells into the cell culture medium (1,2). The pGLuc-Basic 2 Vector contains a multiple cloning site (MCS) upstream of the GLuc coding sequence. A neomycin resistance gene under the control of an SV40 promoter allows selection for stable integration of the plasmid into the mammalian cell genome using G418.
- Polylinker MCS: 12–68
- GLuc coding: 76–633
- Start codon: 76–78
- Stop codon: 631–633
- Signal peptide: 76–126
- Synthetic poly-A site: 642–690
- Neo promoter (SV40): 1276–1611
- Neomycin resistance gene: 1663–2457
- Bacterial replication ori (pMB1): 3791–3203
- Amp resistance: 4822–3962
- All pGLuc 2 vectors and plasmids have improved polyadenylation-transcription termination of the luciferase transcript. The polyadenylation signal is a synthetic polyadenylation sequence based on the β-globin gene (5)
Product SourceIsolated from an E. coli strain NEB10b by standard DNA purification procedure.
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