pCMV-GLuc 2 Control Plasmid
The pCMV-GLuc 2 Control Plasmid is a mammalian expression plasmid that constitutively expresses the secreted Gaussia luciferase (GLuc) under the control of the CMV promoter.
- Strong constitutive expression of GLuc
- GLuc is codon-optimized for mammalian expression
- GLuc activity can be assayed in the culture media or cell lysate
- Can be used to generate Neomycin resistance stable cell lines
The pCMV-GLuc 2 Control Plasmid is a mammalian expression vector that encodes the secreted luciferase from the copepod Gaussia princeps as a reporter, under the control of the constitutive CMV (cytomegalovirus) promoter. Gaussia luciferase (GLuc) is a 19 kDa protein encoded by a "humanized" sequence, and it contains a native signal peptide at the N-terminus that allows it to be secreted from mammalian cells into the cell culture medium (1,2). A neomycin resistance gene under the control of an SV40 promoter allows selection for stable integration of the plasmid into the mammalian cell genome using G418.
- CMV promoter: 209–863
- GLuc coding: 920–1477
- Start codon: 920–922
- Stop codon: 1475–1477
- Signal peptide: 920–970
- Synthetic poly-A site: 1486–1534
- Neo promoter (SV40): 2120–2455
- Neomycin resistance gene: 2507–3301
- Bacterial replication ori (pMB1): 4635–4047
- Amp resistance: 5666–4806
- All pGLuc-2 vectors have improved polyadenylation-transcription termination of the luciferase transcript. The polyadenylation signal is a synthetic polyadenylation sequence based on the β-globin gene (5).
Product SourceIsolated from E. coli strain NEB10β by a standard DNA purification procedure.
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