pCMV-GLuc 2 Control Plasmid

The pCMV-GLuc 2 Control Plasmid is a mammalian expression plasmid that constitutively expresses the secreted Gaussia luciferase (GLuc) under the control of the CMV promoter.

  • Strong constitutive expression of GLuc
  • GLuc is codon-optimized for mammalian expression
  • GLuc activity can be assayed in the culture media or cell lysate
  • Can be used to generate Neomycin resistance stable cell lines

Ordering Information

$name
  • 500 μg/ml
    20 μg each
    $308.00
  • Product Information
    The pCMV-GLuc 2 Control Plasmid is a mammalian expression vector that encodes the secreted luciferase from the copepod Gaussia princeps as a reporter, under the control of the constitutive CMV (cytomegalovirus) promoter. Gaussia luciferase (GLuc) is a 19 kDa protein encoded by a "humanized" sequence, and it contains a native signal peptide at the N-terminus that allows it to be secreted from mammalian cells into the cell culture medium (1,2). A neomycin resistance gene under the control of an SV40 promoter allows selection for stable integration of the plasmid into the mammalian cell genome using G418.

    Comparison of light output obtained from secreted GLuc versus Renilla luciferase in U2OS cells that were transiently transfected with the corresponding expression vectors. Supernatants (GLuc) and lysates (Renilla) were harvested at 24 hour post-transfection and assayed for luciferase activity using the BioLux GLuc Assay System.

    DNASU and Addgene are central repositories for plasmid clones and collections that may also be helpful.

    Highlights

    • CMV promoter: 209–863
    • GLuc coding: 920–1477
    • Start codon: 920–922
    • Stop codon: 1475–1477
    • Signal peptide: 920–970
    • Synthetic poly-A site: 1486–1534
    • Neo promoter (SV40): 2120–2455
    • Neomycin resistance gene: 2507–3301
    • Bacterial replication ori (pMB1): 4635–4047
    • Amp resistance: 5666–4806
    • All pGLuc-2 vectors have improved polyadenylation-transcription termination of the luciferase transcript. The polyadenylation signal is a synthetic polyadenylation sequence based on the β-globin gene (5).

    Product Source

    Isolated from E. coli strain NEB10β by a standard DNA purification procedure.
    N8081_updtSNAP
    Product Categories:
    Gaussia Luciferase Products,
    DNA Plasmid & Substrates Products
    Applications:
    Gaussia Luciferase
    • Advantages and Features
    • Properties and Usage
    • Related Products
    • Notes
    • References
  • FAQs & Tech Tips
  • Protocols & Manuals
  • Other Tools & Resources
  • Quality & Safety
  • Legal Information
    • Legal And Disclaimers
Loading Spinner