Biotinylated 2-Log DNA Ladder (0.1-10 kb)

  • This product was discontinued on 08/28/2014
Catalog #SizeConcentrationGel LanesPriceQtyAdd to Cart
  
Categories:
Discontinued Products

Description

A number of proprietary plasmids are digested to completion with appropriate restriction enzymes to yield 19 bands and subsequently biotinylated for use as molecular weight standards for chemiluminescent detection. All bands have equal intensity except for the 0.5, 1.0 and 3.0 kb bands which have increased intensity to serve as reference points.




N3200_thumb

1.0 µg of 2-Log DNA Ladder visualized by ethidium bromide staining on a 1.0% TBE agarose gel.

Properties and Usage

Bases

FragmentMassbp
14010002
2408001
3486001
4405001
5324001
61203001
7402017
8571517
9451200
101221000
1134900
1231800
1327700
1423600
15a124517
15b500
1649400
1737300
1832200
1961100

Effective Size Range

100bp to 10,002bp

Storage Temperature

-20°C

Storage Conditions

10 mM Tris-HCl
1 mM EDTA
pH 8.0 @ 25°C

Notes

  1. 1 µl of marker per gel lane is usually sufficient to view the DNA fragments using standard chemiluminescent detection.

FAQs

  1. Why are the DNA ladders showing up on my Southern blot? What is the sequence or composition of the ladder bands?
  2. How can I quantify the amount of DNA in each band of a marker?

Datacards

The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.

Selection Charts

Interactive Tools

Safety Data Sheet

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Datacards

The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.