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  • Biotinylated 2-Log DNA Ladder (0.1-10 kb)

    Description

    A number of proprietary plasmids are digested to completion with appropriate restriction enzymes to yield 19 bands and subsequently biotinylated for use as molecular weight standards for chemiluminescent detection. All bands have equal intensity except for the 0.5, 1.0 and 3.0 kb bands which have increased intensity to serve as reference points.




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    1.0 µg of 2-Log DNA Ladder visualized by ethidium bromide staining on a 1.0% TBE agarose gel.

    Properties and Usage

    Bases

    FragmentMassbp
    14010002
    2408001
    3486001
    4405001
    5324001
    61203001
    7402017
    8571517
    9451200
    101221000
    1134900
    1231800
    1327700
    1423600
    15a124517
    15b500
    1649400
    1737300
    1832200
    1961100

    Effective Size Range

    100bp to 10,002bp

    Storage Temperature

    -20°C

    Storage Conditions

    10 mM Tris-HCl
    1 mM EDTA
    pH 8.0 @ 25°C

    Notes

    1. 1 µl of marker per gel lane is usually sufficient to view the DNA fragments using standard chemiluminescent detection.

    Supporting Documents

    Material Safety Datasheets

    The following is a list of Material Safety Data Sheets (MSDS) that apply to this product to help you use it safely. The following file naming structure is used to name these document files: [Product Name] MSDS. For international versions please contact us at info@neb.com.

    Datacards

    The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.
    1. Why are the DNA ladders showing up on my Southern blot? What is the sequence or composition of the ladder bands?
    2. How can I quantify the amount of DNA in each band of a marker?
    3. Can I use SYBR® with the DNA Ladders from NEB?

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