pMYB5 Control Plasmid
- Catalog # N6906 was discontinued on November 01, 2012 ;
pMYB5 is a control plasmid for the IMPACT™ Kit (NEB #E6901) (1,2). This plasmid carries the E. coli malE gene, encoding the maltose binding protein (MBP)(3), fused in-frame to the coding region of the Sce VMA intein-chitin binding domain (55 kDa)(1,4). pMYB5 can be used to test plasmid transformation, cell culture, induction and purification procedures. After induction with 0.3 mM IPTG at 30°C for 3 hours (or 15°C for 12-16 hours), 100 ml of cells should yield 2-3 mg of a 97 kDa fusion protein. After chitin column purification and cleavage, approximately 1.0-1.5 mg of the MBP (42 kDa) is usually obtained. This double stranded vector is 8602 bp in length.
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