pMXB10 Control Plasmid
- This product was discontinued on November 01, 2012
pMXB10 is a control plasmid for the IMPACT™ Kit (NEB #E6901) (1,2). This plasmid carries the E. coli malE gene, encoding the maltose binding protein (MBP, 42 kDa) (3), fused in-frame to the coding region of the Mxe gyrase A intein-chitin binding domain (28 kDa) from pTXB1 (2,4,5). pMXB10 can be used to test plasmid transformation, cell culture, induction and purification procedures. After induction with 0.3 mM IPTG at 30°C for 3 hours (or 15°C for 12-16 hours), 100 ml of cells should yield 2-3 mg of a 70 kDa fusion protein. After chitin column purification and cleavage, approximately 1.5-2.0 mg of MBP is usually obtained. This double stranded vector is 7813 bp in length.
- Expression of the fusion gene is under the control of an IPTG -inducible T7 promoter (5).
- T7 Express Competent E. coli (High Efficiency) (NEB #C2566) or BL21(DE3) Competent E. coli (NEB #C2527) and derivatives can be used as expression hosts.
- A pTXB1 derivative
- Ampicillin resistance
- Product Categories:
- Discontinued Products
- FAQs & Tech Tips
- Other Tools & Resources
- Quality & Safety
- Legal Information
Ineligible item added to cart
Based on your Freezer Program type, you are trying to add a product to your cart that is either not allowed or not allowed with the existing contents of your cart. Please review and update your order accordingly If you have any questions, please contact Customer Service at email@example.com or 1-800-632-5227 x 8.