- Catalog # N6701 was discontinued on November 01, 2012
pTYB1 is an E. coli cloning and expression vector (7477 bp) used in the IMPACT™ Kit (NEB #E6901) which allows the overexpression of a target protein as a fusion to a self-cleavable affinity tag (1). This C-terminal fusion vector is designed for the in-frame insertion of a target gene into a polylinker upstream of an intein tag (the Sce VMA intein/chitin binding domain, 55 kDa) (1,2). This results in the fusion of the C-terminus of the target protein to the N-terminus of the intein tag. Thiol-induced self-cleavage of the intein releases the target protein from the chitin-bound intein tag, resulting in a single column purification of the target protein (1).
For fusion of the N-terminus of the target protein to the intein tag, use pTYB21 (NEB #N6709 ) or pTYB22 (NEB #N6710 ).
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