Universal USER® Cassette

  • Catalog # N5504 was discontinued on January 01, 2013 ;
  • Product Information
    The Universal USER Cassette enables the construction of USER compatible vectors by ligation of an unphosphorylated double-stranded oligonucleotide cassette into any blunt-ended phosphorylated vector. Once the cassette is ligated into the vector of choice, this vector is digested with nicking enzyme Nt.BbvCI and the restriction enzyme XbaI which creates DNA overhangs compatible with the USER processed PCR product described in the USER Friendly PCR Cloning Kit NEB #E5500).



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