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  • Universal USER Cassette

    Discontinued Date

    01/01/2013
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    Categories:
    Discontinued Products

    Description

    The Universal USER Cassette enables the construction of USER compatible vectors by ligation of an unphosphorylated double-stranded oligonucleotide cassette into any blunt-ended phosphorylated vector. Once the cassette is ligated into the vector of choice, this vector is digested with nicking enzyme Nt.BbvCI and the restriction enzyme XbaI which creates DNA overhangs compatible with the USER processed PCR product described in the USER Friendly PCR Cloning Kit NEB #E5500).



    Properties and Usage

    Usage Concentration

    2.5 pmol/μl

    Storage Temperature

    -20°C

    Quality Control

    Quality Assurance Statement

    • The DNA sequence of the cassette was confirmed by DNA sequencing after cloning the Universal USER Cassette into LITMUS 38 digested with StuI. Using either the High Concentration T4 DNA Ligase Reaction protocol or Quick Ligation Reaction protocol outlined below, 20-40% of transformants had the cassette inserted into the vector.

    Notes

    1. Reagents Supplied: Preannealed, unphosphorylated oligonucleotides (50 pmol, 2.5 pmol/µl) in 20 µl of 10 mM Tris-HCl, 1 mM EDTA (pH 8.0).

    Supporting Documents

    Material Safety Datasheets

    The following is a list of Material Safety Data Sheets (MSDS) that apply to this product to help you use it safely. The following file naming structure is used to name these document files: [Product Name] MSDS. For international versions please contact us at info@neb.com.

    Datacards

    The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.
    1. Universal USER Cassette Protocol 2: USER-Friendly Vector Preparation
    2. Universal USER Cassette Protocol 1: Cassette Cloning