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  • pNEB206A Linearized Vector


    Linearized pNEB206A Vector is designed for cloning with USER™. pNEB206A has been linearized within the Multiple Cloning Site to produce 8-nucleotide, 3´ single-stranded extensions on both vector ends. The single-stranded extensions are not complementary; this prevents the vector termini from re-annealing to form transformable circular DNA and also allows control over the orientation of the inserted PCR product. pNEB206A linearized vector is 2,706 base pairs in length.

    DNASU is a central repository for plasmid clones and collections that may also be helpful.

    Properties and Usage

    Storage Temperature


    Quality Control

    Quality Control Assays

    The following Quality Control Tests are performed on each new lot and meet the specifications designated for the product. Individual lot data can be found on the Product Summary Sheet/Datacard or Manual which can be found in the Supporting Documents section of this page.
    • Functional Test (USER Enzyme):
      The USER™ (uracil-specific excision reagent) enzyme is tested for its ability to nick a PCR product at a uracil contained in the primers and assemble with a vector containing complementary overlaps; transformation and plating results in >95% white colonies containing insert.

    Supporting Documents

    Material Safety Datasheets

    The following is a list of Material Safety Data Sheets (MSDS) that apply to this product to help you use it safely. The following file naming structure is used to name these document files: [Product Name] MSDS. For international versions please contact us at info@neb.com.


    The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.

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