CpG Methylated HeLa Genomic DNA
Human female HeLa (cervix adenocarcinoma) genomic DNA that was enzymatically methylated with CpG Methylase (M.SssI), suitable as a positive control in the study of CpG dinucleotide methylation.
HeLa (cervix adenocarcinoma) cells were grown to confluency in DMEM plus 10% fetal bovine serum. Genomic DNA was isolated by a standard genomic purification protocol (1), treated with CpG Methylase (M. SssI), phenol extracted and equilibrated to 10 mM Tris-HCl (pH 7.5) and 1 mM EDTA.
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