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Home Discontinued Products CpG Methylated NIH 3T3 Mouse Genomic DNA

CpG Methylated NIH 3T3 Mouse Genomic DNA

  • Catalog # N4005 was discontinued on January 05, 2015 ;
  • Product Information
    NIH 3T3 (mouse embryonic fibroblast cell line) genomic DNA that was enzymatically methylated with CpG Methylase (M.SssI), suitable as a positive control in the study of CpG dinucleotide methylation.
    Product Categories:
    Discontinued Products
    • Advantages and Features

      Application Features

      • A positive control for Methylation-Specific PCR (MSP) (2), Bisulfite sequencing, Methylation-sensitive Single-Nucleotide Primer Extension (Ms-SNuPE), Combined Bisulfite Restriciton Analysis (COBRA), Bisulfite treatment and PCR-Single-Strand Conformation Polymorphism Analysis (Bisulfite-PCR-SSCP/BiPS).
    • Properties & Usage

      Storage Temperature

      -20°C
    • Product Notes
      1. A 260/280 Ratio: 1.90
    • References
      1. Sambrook, J. and Russell, D. (2001). Molecular Cloning: A Laboratory Manual. (3rd ed.), (pp. 6.4-6.12). Cold Spring Harbor: Cold Spring Ha.
      2. Herman,J. G. and Baylin, S. B. (1996). U.S. Patent No. 5,786,146, Johns Hopkins University School of Medicine.
      3. Frommer, M., et. al. (1992). PNAS USA. 89, 1827-1831.
  • Protocols, Manuals & Usage
  • Quality, Safety & Legal
    • Quality Control Assays
      Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
    • Safety Data Sheets
      The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.
    • Datacards
      The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at [email protected] or fill out the Technical Support Form for appropriate document.)
    • Legal and Disclaimers
      This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).

      While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.

      For more information about commercial rights, please contact NEB's Global Business Development team at [email protected].

      This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

      New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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