5-Aza-dc Treated Jurkat Genomic DNA
Genomic DNA purified from human male Jurkat (human acute T-cell leukemia) cells that are treated with 5-aza-2-deoxycytidine (5-Aza-dc), suitable as a negative control in the study of CpG dinucleotide methylation in the genome.
Jurkat (acute T-cell leukemia) cells were grown to 50% confluency in RPMI plus 10% fetal bovine serum and were treated with a 2 µM 5-aza-2´-deoxycytidine for eight days. Genomic DNA was isolated by a standard genomic purification protocol (1), phenol/chloroform extracted and equilibrated to 10 mM Tris-HCl (pH 7.5) and 1 mM EDTA.
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