CpG Methylated Jurkat Genomic DNA
Human male Jurkat (human acute T-cell leukemia) genomic DNA that was enzymatically methylated with CpG Methylase (M.SssI), suitable as a positive control in the study of CpG dinucleotide methylation.
Jurkat (acute T-cell leukemia) cells were grown to confluency in RPMI plus 10% fetal bovine serum. Genomic DNA was isolated by a standard genomic purification protocol, treated with CpG Methylase (M. SssI), phenol extracted and equilibrated to 10 mM Tris-HCl (pH 7.5) and 1 mM EDTA.
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