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Home Discontinued Products pKLAC1-malE Control Plasmid

pKLAC1-malE Control Plasmid

  • Catalog # N3741 was discontinued on January 01, 2013 ;
Learn more about how this product is being used in the Product Citation Tool
2019-20_Catalog_Promo_0519
  • Product Information

    pKLAC1-malE is a control plasmid for the K. lactis Expression Kit (NEB #E1000). The backbone integrative expression vector, pKLAC1, directs high-level expression of recombinant protein from the yeast Kluyveromyces lactis. Vector pKLAC1-malE carries the E. coli malE gene, encoding the maltose binding protein (MBP). pKLAC1-malE can be used to test plasmid transformation, cell culture, and protein production procedures. A typical K. lactis GG799 strain containing integrated pKLAC1-malE will produce > 25 mg/L of MBP (Figure 1).

    Figure 1: SDS-polyacrylamide gel electrophoresis of secreted recombinant maltose binding protein (MBP). (A) Detection of secreted MBP by Coomassie staining. Lane 1: Protein Molecular Weight Markers. Lane 2: spent culture medium (15 µl) from wild-type K. lactis cells. Lane 3: spent culture medium (15 µl) from K. lactis cells harboring integrated pKLAC1-malE.(B) Detection of secreted MBP by Western analysis using a polyclonal anti-MBP antibody (NEB #E8031S; 1:2000 dilution) and an anti-rabbit IgG-HRP secondary antibody (1:2000 dilution). Lane 1: culture medium from K. lactis cells (15 µl). Lane 2: culture medium (15 µl) from K. lactis cells containing integrated pKLAC1-malE.

    Highlights

    • Expression of the fusion gene is under the control of the strong K. lactis PLAC4-PBI promoter
    • K. lactis strain GG799 can be used as an expression host
    • Ampicillin resistance for propagation in E. coli (See notes for strain recommendations.)
    • Fungal acetamidase selectable marker for integration into the K. lactis genome
    Product Categories:
    Discontinued Products
  • Protocols, Manuals & Usage
  • Tools & Resources
  • FAQs & Troubleshooting
    • Tech Tips
      Always do the control transformation. This will help mediate any downstream expression issues which may arise.
  • Citations & Technical Literature
  • Quality, Safety & Legal
    • Quality Control Assays
      Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
    • Safety Data Sheets
      The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.
    • Datacards
      The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at [email protected] or fill out the Technical Support Form for appropriate document.)
    • Legal and Disclaimers
      This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).

      While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.

      For more information about commercial rights, please contact NEB's Global Business Development team at [email protected].

      This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

      Licenses

      The vector pKLAC1-malE is a component of an expression system that was developed from basic research at New England Biolabs, Inc. and DSM Biologics Company B.V. The buyer/user has a non-exclusive sublicense to use this system or any component thereof, including vector pKLAC1-malE, for RESEARCH PURPOSES ONLY. A license to use this system for manufacture of clinical grade material or commercial purposes is available from New England Biolabs, Inc., or DSM Biologics Company B.V.

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