LITMUS™ U
- This product was discontinued on January 01, 2013
Ordering Information
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Product Information
LITMUS™ U is a linear vector with 8 nucleotide, 3´non-complementary overhangs that can be used for the rapid cloning of PCR fragments without ligation (Figure 1). LITMUS U has opposing T7 promoters which generate dsRNA from a cloned fragment with little additional sequence by in vitro transcription with T7 RNA Polymerase.
To clone into LITMUS U, PCR products must be generated using primers that have the following sequences at the 5´ end: 5´ GGAGACAUNNN... and 5´ GGGAAAGUNNN... (U = uracil and NNN... = specific primer sequence). After PCR, the DNA product can be directly cloned into LITMUS U using the USER Enzyme (NEB #M5505 ) in a 30 minute reaction without ligation or any purification steps (1) following the USER Friendly Cloning Kit (NEB #E5500) protocol.
Figure 1: Cloning a PCR Product into LITMUS U. Highlights
- Fast, simple & extremely efficient cloning of PCR Products
- Mix PCR product with USER™ Enzyme and vector, incubate for 30 minutes, transform
- No PCR product cleanup required
- Product Categories:
- Discontinued Products
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Protocols & Manuals
- Protocols
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Datacards
The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.)
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Other Tools & Resources
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Quality & Safety
- Quality Control Assays
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Safety Data Sheets
The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.
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Datacards
The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.)
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Legal Information
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