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Home Discontinued Products MidRange II PFG Marker

MidRange II PFG Marker

  • This product was discontinued on January 01, 2013

Ordering Information

N3552
  • Discontinued
    Discontinued
  • Product Information
    MidRange Marker II is concatemers of λ DNA isolated from the bacteriophage λ (cI857 ind1 Sam7) mixed with XbaI digested λ DNA embedded in 1% LMP agarose and supplied in a GelSyringe™ dispenser. XbaI produces fragments of 24.0 and 24.5 kb. These fragments anneal to and form concatamers with intact λ DNA. It is designed for use as a size marker for pulsed field gel electrophoresis (PFG). Size range: 24-300 kb.


    N3552_thumb
    Product Categories:
    Discontinued
    • Properties and Usage

      Bases

      Fragment bp
      1 24000
      2 24500
      3 48500
      4 72500
      5 73000
      6 97000
      7 121000
      8 121500
      9 145500
      10 169500
      11 170000
      12 194000
      13 218000
      14 218500
      15 242500
      16 266500
      17 267000
      18 291000

      Effective Size Range

      24,000bp to 291,000bp

      Storage Temperature

      -20°C

      Storage Conditions

      10 mM Tris-HCl
      1 mM EDTA
      50% Glycerol
      1% LMP agarose
      pH 8.0 @ 25°C

    • Related Products

      Companion Products

    • Notes
      1. Recommended plug sizes are from 5-10 µl. A 10 µl plug (one small graduation on the GelSyringe volume scale) contains approximately 0.5 µg of DNA. Each GelSyringe yields 50+ plugs.
      2. Melting plugs will cause denaturation of concatemers.
      3. Extrude agarose from GelSyringe carefully and slice plugs from the end with a sharp blade. One plug is sufficient for one lane of a gel. Place the plug at the front of the well and seal with molten agarose. Allow no bubbles to form.
      4. Place plug at the front of the well and seal with molten agarose just above gelling temperature(~42–45°C). Allow no bubbles to form. Never attach the agarose plugs to the gel comb before the gel is poured. Heat from the solidifying gel will cause the Lambda concatemers to denature.
      5. The photograph represents the pulsed field gel separation of Mid Range II Markers using a CHEF apparatus. The 1% agarose gel was run at 6 volts/cm using ramped pulse times from 1 to 25 seconds for 24 hours at 15°C in 0.5X TBE (50 mM Tris-HCl, 50 mM boric acid, 1 mM EDTA) made with Milli-Q™ water.
    • References
      1. Greci, J. and Davis, T.B., New England Biolabs, unpublished observation.
  • FAQs & Tech Tips
  • Quality & Safety
    • Quality Control Assays
      Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
    • Safety Data Sheets
      The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.
  • Legal Information
    • Legal And Disclaimers
      This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).

      While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.

      For more information about commercial rights, please contact NEB's Global Business Development team at gbd@neb.com.

      This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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