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  • TriDye™ 2-Log DNA Ladder (0.1 - 10.0 kb)

    Description

    TriDye™ 2-Log DNA Ladder is a pre-mixed, ready-to-load molecular weight marker containing 3 dyes which serve as visual aids to monitor the progress of migration during agarose gel electrophoresis.

    The DNA Ladder consists of proprietary plasmids which are digested to completion with appropriate restriction enzymes to yield 19 bands suitable for use as molecular weight standards for agarose gel electrophoresis. This digested DNA includes fragments ranging from 100 bp to 10 kb. The 0.5, 1.0 and 3.0 kb bands have increased intensity to serve as reference points.

    TriDye During Electrophoresis: On a standard 1% agarose gel in 1X TBE, xylene cyanol FF migrates at approximately 4 kb, bromophenol blue at approximately 300 bp and the orange G at approximately 50 bp. As the percentage of agarose changes, the migration rates of the dyes relative to migration rates of the DNA will change.


    N3270fig2_TN

    2-Log DNA Ladder visualized by ethidium bromide staining on a 1.0% TBE agarose gel. Mass values are for 1 µg/lane.

    Properties and Usage

    Bases

    FragmentMassbp
    14010002
    2408001
    3486001
    4405001
    5324001
    61203001
    7402017
    8571517
    9451200
    101221000
    1134900
    1231800
    1327700
    1423600
    15124500
    1649400
    1737300
    1832200
    1961100

    Effective Size Range

    100bp to 10,002bp

    Storage Temperature

    4°C

    Storage Conditions

    0.006% Xylene Cyanol
    10 mM Tris-HCl
    10 mM EDTA
    10% Glycerol
    0.006% bromophenol blue
    0.06% Orange G
    pH 7.9 @ 25°C

    Notes

    1. The TriDye DNA Ladder was not designed for precise quantification of DNA mass but can be used for approximating the mass of DNA in comparably intense samples of similar size.
    2. We recommend loading 5-10 μl (0.5-1.0 μg) of TriDye DNA ladder per gel lane.
    3. In general, it is recommended to load 1ul per mm of gel lane.
    4. TriDye DNA Ladder is stable for at least 6 months at 25°C.
    5. For long term storage, store at 4°C or -20°C. If stored at -20°C, mix well after thawing.

    References

    1. Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989). Molecular Cloning: A Laboratory Manual (2nd Ed.). 10.51-10.67.

    FAQs

    1. Why are the DNA ladders showing up on my Southern blot? What is the sequence or composition of the ladder bands?
    2. How can I quantify the amount of DNA in each band of a marker?
    3. Can I use GelRed with the DNA Ladders from NEB?
    4. Can I use Midori Green with the DNA Ladders from NEB?
    5. Can I use SYBR® with the DNA Ladders from NEB?

    Tech Tips

    Mix well before use, especially after thawing.

    Datacards

    The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.

    Selection Tools

    Interactive Tools

    Certificate of Analysis

    The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality control's for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

    Specifications

    The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

    Safety Data Sheet

    The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

    Datacards

    The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.