Mass DNA Ladder

  • This product was discontinued on 12/03/2014
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Discontinued Products

Description

The Mass DNA ladder is a pre-mixed, ready-to-load molecular weight marker containing Orange G dye as a tracking dye. 

The DNA Ladder consists of proprietary plasmids, which are digested to completion with appropriate restriction enzymes to yield 7 bands suitable for size determination and mass quantification of linear double-stranded DNA fragments. The digested DNA includes fragments ranging from 0.5 to 10 kilobases.

Comes supplied with 1 vial of Gel Loading Dye, Orange (6X).



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Mass DNA Ladder. Mass values are for .75 μg/lane

Reagents Supplied

The following reagents are supplied with this product:

Store at (°C)Concentration
Gel Loading Dye, Orange (6X)256X

Properties and Usage

Bases

FragmentMassbp
124010002
21806001
31204001
4753001
5602017
6451008
730500

Preparation

The double-stranded DNA is digested to completion with appropriate restriction enzymes, phenol extracted and equilibrated in storage buffer.

Effective Size Range

500bp to 10,002bp

Storage Temperature

4°C

Storage Conditions

3.3 mM Tris-HCl
11 mM EDTA
0.15% Orange G
0.017% SDS
2.5% Ficoll®-400
pH 8.0 @ 25°C

Notes

  1. The 500bp band comprises 4 DNA fragments ranging in size between 494 and 517bp.

  2. Preparation: The double-stranded DNA is digested to completion with appropriate restriction enzymes, phenol extracted and equilibrated in storage buffer.

  3. Usage Recommendation: We recommend loading 5 to 20 µl (0.25 µg to 1 µg) of the Mass DNA Ladder per gel lane. For a more accurate mass estimation, always compare the samples intensity to the ladder’s band of nearest size. The loading volume and the loading buffer for the samples and the Mass DNA Ladder should be the same whenever possible.

  4. Mass DNA Ladder is stable for at least 6 months at 25°C.

  5. For long term storage. Store at 4°C or -20°C. If stored at -20°C, mix well after thawing.

References

  1. Sambrook, J., Fritsch, E. F. and Maniatis, T. (1989). Cold Spring Harbor(Ed.), 10.51-10.67.

FAQs

  1. How much Mass DNA Ladder should I load on a gel?
  2. Can I store it at -20°C?
  3. Can I use GelRed with the DNA Ladders from NEB?
  4. Can I use SYBR® with the DNA Ladders from NEB?

Datacards

The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.

Selection Charts

Interactive Tools

Certificate of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality control's for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Safety Data Sheet

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Datacards

The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.