pKLAC1-GLuc Control Plasmid
- This product was discontinued on January 01, 2013
pKLAC1-GLuc is a yeast reporter plasmid that can be used with the K. lactis Expression Kit (NEB #E1000). The backbone integrative expression vector, pKLAC1, directs high-level secretion of Gaussia priniceps luciferase (GLuc) from the yeast Kluyveromyces lactis. The gene encoding GLuc is cloned in the NotI-StuI sites of pKLAC1, in-frame with the a-factor secretion leader domain. pKLAC1-GLuc can be used to test plasmid transformation, cell culture, and protein production procedures.
Additionally, GLuc can be used as a carboxy-terminal fusion tag on a desired protein to track its secretion. GLuc activity is easily detected in K. lactis spent culture medium (Figure 1) using the Gaussia Luciferase Assay Kit (NEB #E3300).
- Product Categories:
- Discontinued Products
- Other Tools & Resources
- Quality & Safety
- Legal Information
Ineligible item added to cart
Based on your Freezer Program type, you are trying to add a product to your cart that is either not allowed or not allowed with the existing contents of your cart. Please review and update your order accordingly If you have any questions, please contact Customer Service at firstname.lastname@example.org or 1-800-632-5227 x 8.