pKLAC1-GLuc Control Plasmid

  • This product was discontinued on 01/01/2013

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N3034
  • Discontinued
    Discontinued
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    pKLAC1-GLuc is a yeast reporter plasmid that can be used with the K. lactis Expression Kit (NEB #E1000). The backbone integrative expression vector, pKLAC1, directs high-level secretion of Gaussia priniceps luciferase (GLuc) from the yeast Kluyveromyces lactis. The gene encoding GLuc is cloned in the NotI-StuI sites of pKLAC1, in-frame with the a-factor secretion leader domain. pKLAC1-GLuc can be used to test plasmid transformation, cell culture, and protein production procedures.

    Additionally, GLuc can be used as a carboxy-terminal fusion tag on a desired protein to track its secretion. GLuc activity is easily detected in K. lactis spent culture medium (Figure 1) using the Gaussia Luciferase Assay Kit (NEB #E3300).


    Figure 1. Detection of a secreted malE-Gluc fusion protein in spent culture medium. Medium (25 µl) from wild-type GG799 cells (lane 1) and GG799 cells contaning integrated pKLAC1-malE-Gluc was assyed using the Gaussia Luciferase Assay Kit (NEB #E3300S ).

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