NEBlot Kit is tested for its ability to label 25 ng of HindIII digested lambda DNA to a specific activity of > 1 x 109 dpm/µg.
The following reagents are supplied with this product:
|Store at (°C)||Concentration|
|Nuclease Free dH2O|
|Klenow Fragment (3'→5' exo-)||-20||5,000 units/ml|
|dATP Solution||-20||0.5 mM|
|dGTP Solution||-20||0.5 mM|
|Octadeoxyribonucleotides in 10X Labeling Buffer||400 µg/ml|
|Control DNA, HindIII digested lambda DNA||25 µg/ml|
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