Quick-Load® Purple 2-Log DNA Ladder (0.1 - 10.0 kb)

Description

Quick-Load Purple 2-Log DNA Ladder is a pre-mixed, ready-to-load molecular weight marker containing one tracking dye. Tracking dye does not leave a shadow under UV light.

Source
Quick-Load Purple 2-Log DNA Ladder consists of proprietary plasmids, which are digested to completion with appropriate restriction enzymes to yield 19 bands suitable for use as molecular weight standards for agarose gel electrophoresis. The digested DNA includes fragments ranging from 100 bp to 10 kb. The 0.5, 1.0 and 3.0 kb bands have increased intensity to serve as reference bands.

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2-Log DNA Ladder visualized by ethidium bromide staining on a 1.0% TBE agarose gel.
Mass values are for 1 µg/lane.

Suggested Load: 10 µl/lane.


N0550_DNALadder_UVcomparison

UV Shadow Comparison
The new Gel Loading Dye, Purple (6X) (Lane 1) included in the Quick-Load Purple 2-log DNA Ladder does not cast a UV shadow over the underlying bands, unlike the Gel Loading Dye, Blue (6X) (Lane 2).

Reagents Supplied

The following reagents are supplied with this product:

Store at (°C)Concentration
Gel Loading Dye, Purple (6X), no SDS256X

Properties and Usage

Bases

FragmentMassbp
14010,002
2408,001
3486,001
4405,001
5324,001
61203,001
7402,017
8571,517
9451,200
101221,000
1134900
1231800
1327700
1423600
15124500/517
1649400
1737300
1832200
1961100

Effective Size Range

100bp to 10,002bp

Storage Temperature

4°C

Storage Conditions

2.5% Ficoll®-400
10 mM EDTA
3.3 mM Tris-HCl
0.001% Dye 2
0.02% Dye 1
pH 8.0 @ 25°C

Notes

  1. Preparation of DNA
    The double-stranded DNA is digested to completion with appropriate restriction enzymes, phenol extracted and equilibrated in storage buffer.

    Usage recommendation
    We recommend loading 5-10 ul (0.5-1 ug) of Quick-Load Purple 2-Log DNA Ladder per gel lane. The Quick-Load Purple 2-Log DNA Ladder is not intended for precise quantification of DNA mass but can be used for approximating the mass of DNA in comparably intense samples of similar size.
  2. Quick-Load Purple 2-Log DNA Ladder is stable for at least 6 months at 25°C.
    For long term storage, store at 4°C or -20°C.
    If stored at -20°C, mix well after thawing. If not well mixed, the load may float out of the wells.

References

  1. Sambrook, J., Fritsch, E. F. and Maniatis, T. (1989). Molecular cloning: A Laboratory Manual. (2nd ed.), (pp. 10.51-10.67). Cold Spring Harbor: Cold Spring Harbor Laboratory Press.

FAQs

  1. Why are there extra bands visible on polyacrylamide gels?
  2. Can I use GelRed with the DNA Ladders from NEB?
  3. Can I use Midori Green with the DNA Ladders from NEB?
  4. Can I use SYBR® with the DNA Ladders from NEB?

Selection Charts

Interactive Tools

Certificate of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality control's for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Safety Data Sheet

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.