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  • Quick-Load® Purple 2-log DNA Ladder (0.1 - 10.0 kb)

    Description

    Quick-Load Purple 2-log DNA Ladder is a pre-mixed, ready-to-load molecular weight marker containing one tracking dye. Tracking dye does not leave a shadow under UV light.

    Source
    Quick-Load Purple 2-log DNA Ladder consists of proprietary plasmids, which are digested to completion with appropriate restriction enzymes to yield 19 bands suitable for use as molecular weight standards for agarose gel electrophoresis. The digested DNA includes fragments ranging from 100 bp to 10 kb. The 0.5, 1.0 and 3.0 kb bands have increased intensity to serve as reference bands.

    N0550

    Reagents Supplied

    The following reagents are supplied with this product:

    Store at (°C)Concentration
    Gel Loading Dye, Purple (6X)256X

    Properties and Usage

    Bases

    FragmentMassbp
    14010,002
    2408,001
    3486,001
    4405,001
    5324,001
    61203,001
    7402,017
    8571,517
    9451,200
    101221,000
    1134900
    1231800
    1327700
    1423600
    15124500/517
    1649400
    1737300
    1832200
    1961100

    Effective Size Range

    100bp to 10,002bp

    Storage Temperature

    4°C

    Storage Conditions

    2.5% Ficoll®-400
    10 mM EDTA
    3.3 mM Tris-HCl
    0.001% Dye 2
    0.02% Dye 1
    pH 8.0 @ 25°C

    Notes

    1. Preparation of DNA
      The double-stranded DNA is digested to completion with appropriate restriction enzymes, phenol extracted and equilibrated in storage buffer.

      Usage recommendation
      We recommend loading 5-10 ul (0.5-1 ug) of Quick-Load Purple 2-log DNA Ladder per gel lane. The Quick-Load Purple 2-log DNA Ladder is not intended for precise quantification of DNA mass but can be used for approximating the mass of DNA in comparably intense samples of similar size.

    2. Quick-Load Purple 2-log DNA Ladder is stable for at least 6 months at 25°C.
      For long term storage, store at 4°C or -20°C.
      If stored at -20°C, mix well after thawing. If not well mixed, the load may float out of the wells.

    References

    1. Sambrook, J., Fritsch, E. F. and Maniatis, T. (1989). Molecular cloning: A Laboratory Manual. (2nd ed.), (pp. 10.51-10.67). Cold Spring Harbor: Cold Spring Harbor Laboratory Press.

    Supporting Documents

    Material Safety Datasheets

    The following is a list of Material Safety Data Sheets (MSDS) that apply to this product to help you use it safely. The following file naming structure is used to name these document files: [Product Name] MSDS. For international versions please contact us at info@neb.com.
    1. Why are there extra bands visible on polyacrylamide gels?
    2. Can I use GelRed with the DNA Ladders from NEB?
    3. Can I use SYBR® with the DNA Ladders from NEB?
    4. Can I use Midori Green with the DNA Ladders from NEB?

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