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  • Quick-Load® PCR Marker

    Description

    Quick-Load® PCR Marker is a pre-mixed, ready-to-load molecular weight marker containing bromophenol blue as a tracking dye.

    The Quick-Load PCR Marker consists of a proprietary plasmid which is digested to completion with appropriate restriction enzymes to yield 5 double-stranded DNA bands that are suitable for use as molecular weight standards for agarose gel electrophoresis. The digested DNA includes fragments ranging from 50–766 base pairs.

    N0475_thumb

    Quick-Load PCR Marker visualized by ethidium bromide staining on a 1.8% TBE agarose gel. Mass values are for 0.3 µg/lane.

    Properties and Usage

    Bases

    FragmentMassbp
    162766
    240500
    348300
    461150
    58950

    Preparation

    Preparation of DNA: The double-stranded DNA is digested to completion with appropriate restriction enzymes, phenol extracted and equilibrated in storage buffer.

    Effective Size Range

    50bp to 766bp

    Storage Temperature

    4°C

    Storage Conditions

    3.3 mM Tris-HCl
    11 mM EDTA
    0.015% bromophenol blue
    0.017% SDS
    2.5% Ficoll®-400
    pH 8.0 @ 25°C

    Notes

    1. Preparation of DNA: The double-stranded DNA is digested to completion with appropriate restriction enzymes, phenol extracted and equilibrated in storage buffer.
    2. We recommend loading 5-10 μl (0.5-1.0 μg) of Quick-Load DNA Ladder per gel lane.
    3. In general, it is recommended to load 1µl per mm of gel lane.
    4. For long term storage, store at 4°C or -20°C. If stored at -20°C, mix well after thawing.

    References

    1. Sambrook, J., Fritsch, E. F. and Maniatis, T. (1989). Molecular Cloning: A Laboratory Manual. (2nd ed.),pp.10.51-10.67, Cold Spring Harbor Laboratory Press.

    Supporting Documents

    Material Safety Datasheets

    The following is a list of Material Safety Data Sheets (MSDS) that apply to this product to help you use it safely. The following file naming structure is used to name these document files: [Product Name] MSDS. For international versions please contact us at info@neb.com.

    Datacards

    The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.
    1. Why are the DNA ladders showing up on my Southern blot? What is the sequence or composition of the ladder bands?
    2. Why is the separation of the lower bands incomplete?
    3. Can I use GelRed with the DNA Ladders from NEB?
    4. Can I use SYBR® with the DNA Ladders from NEB?
    5. Can I use Midori Green with the DNA Ladders from NEB?

    Selection Tools

    Mix well before use, especially after thawing.