Quick-Load® PCR Marker

An alternative product available for purchase is PCR Marker (N3234).
  • This product was discontinued on 01/05/2015
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Discontinued Products

Description

Quick-Load® PCR Marker is a pre-mixed, ready-to-load molecular weight marker containing bromophenol blue as a tracking dye.

The Quick-Load PCR Marker consists of a proprietary plasmid which is digested to completion with appropriate restriction enzymes to yield 5 double-stranded DNA bands that are suitable for use as molecular weight standards for agarose gel electrophoresis. The digested DNA includes fragments ranging from 50–766 base pairs.

N0475_thumb

Quick-Load PCR Marker visualized by ethidium bromide staining on a 1.8% TBE agarose gel. Mass values are for 0.3 µg/lane.

Properties and Usage

Bases

FragmentMassbp
162766
240500
348300
461150
58950

Preparation

Preparation of DNA: The double-stranded DNA is digested to completion with appropriate restriction enzymes, phenol extracted and equilibrated in storage buffer.

Effective Size Range

50bp to 766bp

Storage Temperature

4°C

Storage Conditions

3.3 mM Tris-HCl
11 mM EDTA
0.015% bromophenol blue
0.017% SDS
2.5% Ficoll®-400
pH 8.0 @ 25°C

Notes

  1. Preparation of DNA: The double-stranded DNA is digested to completion with appropriate restriction enzymes, phenol extracted and equilibrated in storage buffer.
  2. We recommend loading 5-10 μl (0.5-1.0 μg) of Quick-Load DNA Ladder per gel lane.
  3. In general, it is recommended to load 1µl per mm of gel lane.
  4. For long term storage, store at 4°C or -20°C. If stored at -20°C, mix well after thawing.

References

  1. Sambrook, J., Fritsch, E. F. and Maniatis, T. (1989). Molecular Cloning: A Laboratory Manual. (2nd ed.),pp.10.51-10.67, Cold Spring Harbor Laboratory Press.

FAQs

  1. Why are the DNA ladders showing up on my Southern blot? What is the sequence or composition of the ladder bands?
  2. Why is the separation of the lower bands incomplete?
  3. Can I use GelRed with the DNA Ladders from NEB?
  4. Can I use Midori Green with the DNA Ladders from NEB?
  5. Can I use SYBR® with the DNA Ladders from NEB?

Tech Tips

Mix well before use, especially after thawing.

Datacards

The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.

Selection Charts

Certificate of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality control's for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Safety Data Sheet

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Datacards

The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.