• My NEB
  • Print
  • PDF
  • Quick-Load® Low Molecular Weight DNA Ladder

    Description

    Quick-Load® Low Molecular Weight DNA Ladder is a pre-mixed, ready-to-load molecular weight marker containing bromophenol blue as a tracking dye.

    The DNA Ladder consists of a proprietary plasmid which is digested to completion with appropriate restriction enzymes to yield 11 bands suitable for use as molecular weight standards for agarose gel electrophoresis. This digested DNA includes fragments ranging from 25–766 base pairs. The 200 base pair band has increased intensity to serve as a reference point.

    N0474_thumb

    Quick-Load LMW DNA Ladder visualized by ethidium bromide staining on a 3% TBE agarose gel. Mass values are for 0.5 µg/lane.

    Suggested  Load: 10 µl/ gel lane. 

    Properties and Usage

    Bases

    FragmentMassbp
    142766
    227500
    320350
    433300
    527250
    6110200
    733150
    843100
    95875
    106350
    114325

    Effective Size Range

    25bp to 766bp

    Storage Temperature

    4°C

    Storage Conditions

    3.3 mM Tris-HCl
    11 mM EDTA
    0.015% bromophenol blue
    0.017% SDS
    2.5% Ficoll®-400
    pH 8.0 @ 25°C

    Notes

    1. Preparation of DNA: The double-stranded DNA is di­gested to completion with appropriate restriction enzymes, phenol extracted and equilibrated in storage buffer.
    2. Quick-Load Low Molecular Weight DNA Ladder is stable for at least 6 months at 25°C.
    3. In general, it is recommended to load 1µl per mm of gel lane.
    4. For long term storage, store at 4°C or -20°C. If stored at -20°C, mix well after thawing.

    References

    1. Sambrook, J., Fritsch, E. F. and Maniatis, T. (1989). Molecular Cloning: A Laboratory Manual. (2nd ed.),pp.10.51-10.67, Cold Spring Harbor Laboratory Press.

    FAQs

    1. Why are the DNA ladders showing up on my Southern blot? What is the sequence or composition of the ladder bands?
    2. Why are there extra bands visible on polyacrylamide gels?
    3. Why is the separation of the lower bands incomplete?
    4. Can I use GelRed with the DNA Ladders from NEB?
    5. Can I use Midori Green with the DNA Ladders from NEB?
    6. Can I use SYBR® with the DNA Ladders from NEB?

    Tech Tips

    Mix well before use, especially after thawing.

    Datacards

    The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.

    Selection Tools

    Interactive Tools

    Certificate of Analysis

    The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality control's for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

    Specifications

    The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

    Safety Data Sheet

    The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

    Datacards

    The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.