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  • Quick-Load® 50 bp DNA Ladder

    Description

    Quick-Load® 50 bp DNA Ladder is a pre-mixed, ready-to-load molecular weight marker containing bromophenol blue as a tracking dye.

    The DNA Ladder consists of proprietary plasmids which are digested to completion with appropriate restriction enzymes to yield 17 bands suitable for use as molecular weight standards for agarose gel electrophoresis. The digested DNA includes fragments ranging from 50–1,350 base pairs. The 200 and 500 base pair bands have increased intensity to serve as reference points.

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    Quick-Load 50 bp DNA Ladder visualized by ethidium bromide staining on a 3% TBE agarose gel. Mass values are for 1 

    µg/lane.



    Suggested Load: 5-10 

    µl/gel lane. 

    Properties and Usage

    Bases

    FragmentMassbp
    171031350
    1670916
    1558766
    1454700
    1350650
    1246600
    1142550
    1076500
    934450
    831400
    737350
    646300
    557250
    4107200
    346150
    269100
    18450

    Effective Size Range

    50bp to 1,350bp

    Storage Temperature

    4°C

    Storage Conditions

    3.3 mM Tris-HCl
    11 mM EDTA
    0.015% bromophenol blue
    0.017% SDS
    2.5% Ficoll®-400
    pH 8.0 @ 25°C

    Notes

    1. Preparation of DNA: The double-stranded DNA is di­gested to completion with appropriate restriction enzymes, phenol extracted and equilibrated in storage buffer.
    2. We recommend loading 5-10 μl (0.5-1.0 μg) of Quick-Load® 50 bp DNA Ladder per gel lane.
    3. In general, it is recommended to load 1µl per mm of gel lane.
    4. The Quick-Load DNA Ladder is not intended for precise quantification of DNA mass but can be used for approximating the mass of DNA in comparably intense samples of similar size.
    5. Quick-Load DNA Ladder is stable for at least 6 months at 25°C.
    6. For long term storage, store at 4°C or -20°C. If stored at -20°C, mix well after thawing.

    References

    1. Sambrook, J., Fritsch, E. F. and Maniatis, T. (1989). Molecular Cloning: A Laboratory Manual. (2nd ed.) pp. 10.51-10.67, Cold Spring Harbor Laboratory Press.

    FAQs

    1. Why are there extra bands visible on polyacrylamide gels?
    2. Why are the DNA ladders showing up on my Southern blot? What is the sequence or composition of the ladder bands?
    3. Can I use GelRed with the DNA Ladders from NEB?
    4. Can I use Midori Green with the DNA Ladders from NEB?
    5. Can I use SYBR® with the DNA Ladders from NEB?

    Tech Tips

    Mix well before use, especially after thawing.

    Datacards

    The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.

    Selection Tools

    Interactive Tools

    Certificate of Analysis

    The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality control's for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

    Specifications

    The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

    Safety Data Sheet

    The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

    Datacards

    The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.