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  • DNA Gyrase (E. coli) Substrate

    Description

    A double-stranded covalently closed circular DNA molecule produced by incubating supercoiled pUC19 DNA with Topoisomerase I (E. coli).

    Product Source

    pUC19 is isolated from E. coli ER2272 (dam+ dcm+ EcoK M– ) by a standard plasmid purification procedure

    Properties and Usage

    Storage Temperature

    -20°C

    Storage Conditions

    10 mM Tris-HCl
    1 mM EDTA
    pH 8.0 @ -20°C

    References

    1. Yanisch-Perron, C., Vieira, J. and Messing, J. (1985). Gene. 33, 003-119.

    Datacards

    The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.

    Usage Guidelines & Tips

    Safety Data Sheet

    The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

    Datacards

    The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.