• My NEB
  • Print
  • PDF
  • pLox2+ (linearized)

    Description

    pLox2+ (linearized) is a substrate for Cre Recombinase (NEB #M0298). It is 3.65 kb in length and has a loxP site approximately 350 bp from each end. Between the loxP sites lie an origin of replication and an ampicillin resistance gene. Recombination between these loxP sites by Cre Recombinase produces a circular, ampicillin-resistant plasmid (which migrates at approximately 1.7 kb on a 0.8% agarose gel) and a 850 bp DNA fragment.

    Properties and Usage

    Storage Temperature

    -20°C

    References

    1. Cantor, E. and Chong, S. (2001). Prot. Exp. Purif.. 22, 135-140.

    Supporting Documents

    Material Safety Datasheets

    The following is a list of Material Safety Data Sheets (MSDS) that apply to this product to help you use it safely. The following file naming structure is used to name these document files: [Product Name] MSDS. For international versions please contact us at info@neb.com.

    Datacards

    The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.
    1. Is the Cre Recombinase control DNA available as a separate product?

    Interactive Tools