Low Range PFG Marker

  • This product was discontinued on 12/10/2014
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Discontinued Products

Description

A mixture of lambda DNA-HindIII fragments and lambda concatemers embedded in 1% LMP agarose and supplied in a GelSyringe™ dispenser. The lambda DNA monomer (48.5 kb) can be used as a reference point as its concentration has been adjusted to make it the brightest band in the pattern. Designed to be used as size markers for pulsed-field gel electrophoresis (PFG). Size range: 0.1-200 kb.

N0350_thumb

1% agarose gel, 6 V/cm, 15°C for 15 hours. Switch times ramped from 1-12 seconds.

Properties and Usage

Bases

Fragmentbp
12194000
11145500
1097000
948500
823100
79420
66550
54360
42320
32030
2560
1130

Effective Size Range

130bp to 194,000bp

Storage Temperature

-20°C

Storage Conditions

10 mM Tris-HCl
1 mM EDTA
50% Glycerol
1% LMP agarose
pH 8.0 @ 25°C

Notes

  1. The photograph represents the pulsed field gel separation of Low Range Markers using a CHEF apparatus. The 1% agarose gel was run at 6 volts/cm using ramped pulse times from 1 to 12 seconds for 15 hours at 15°C in 0.5X TBE (50 mM Tris-HCl, 50 mM boric acid, 1 mM EDTA) made with Milli-Q™ water.
  2. Bands larger than 194 kb are successively larger concatemers of Lambda DNA.
  3. Extrude agarose from GelSyringe carefully and slice plugs from the end with a sharp blade. One plug is sufficient for one lane of a gel. Place the plug at the front of the well and seal with molten agarose. Allow no bubbles to form.
  4. Recommended plug size is 20 µl (two small graduations on the GelSyringe volume scale) which contains approximately 0.5 µg of DNA. Each GelSyringe yields 25+ plugs.
  5. Melting plugs will cause denaturation of concatemers.
  6. Place plug at the front of the well and seal with molten agarose just above gelling temperature(~42–45°C). Allow no bubbles to form. Never attach the agarose plugs to the gel comb before the gel is poured. Heat from the solidifying gel will cause the Lambda concatemers to denature.

FAQs

  1. There are several factors that lead to poor results for PFG markers. Here is a list of recommendations for ensuring a sharp high quality banding pattern.

Datacards

The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.

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Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Safety Data Sheet

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Datacards

The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.