• My NEB
  • Print
  • PDF
  • Yeast Chromosome PFG Marker

    Description

    Chromosomes isolated from Saccharomyces cerevisiae, strain YPH80 embedded in 1% LMP agarose and supplied in a GelSyringe™ dispenser. Designed for use as size markers for pulsed-field gel electrophoresis (PFG). Size range: 225-1,900 kb. 

    N0345_thumb

    1% agarose gel, 6.0 V/cm, 15°C for 26 hours. Switch time of 70 seconds for 15 hours and 120 seconds for 11 hours.

    Properties and Usage

    Bases

    Fragmentbp
    XII1900000
    IV1640000
    VII1120000
    XV1100000
    XVI945000
    XIII915000
    II815000
    XIV785000
    X745000
    XI680000
    V610000
    VIII555000
    IX450000
    III375000
    VI295000
    I225000

    Effective Size Range

    225,000bp to 1,900,000bp

    Storage Temperature

    -20°C

    Storage Conditions

    0.25 mM EDTA
    50% Glycerol
    1% LMP agarose
    pH 9.0 @ 25°C

    Notes

    1. Extrude agarose from GelSyringe carefully and slice plugs from the end with a sharp blade. One plug is sufficient for one lane of a gel. Place the plug at the front of the well and seal with molten agarose. Allow no bubbles to form.
    2. Recommended plug size is 20 µl (two small graduations on the syringe volume scale), which contains approximately 1 µg of DNA. Each syringe yields 25+ plugs.
    3. The photograph represents the pulsed field gel separation of Yeast Chromosomes using a CHEF apparatus. The 1% agarose gel was run at 6 volts/cm using pulse times of 70 seconds (15 hours) and 120 seconds (11 hours) at 15°C in 0.5X TBE buffer (50 mM Tris-HCl, 50 mM boric acid, 1 mM EDTA) made with Milli-Q™ water, allowing resolution of 15 of the 16 Yeast Chromosomes. Chromosomes VII and XV appear as a single band. Chromosome XII may display anomalous electrophoretic behavior.
    4. Bases Table:  The roman numerals at the left of this table designate chromosome numbers.

    References

    1. Carle, G. and Olson, M. (1985). Proc. Natl. Acad. Sci. USA. 82, 3756.
    2. Mortimer, R. and Schild, D. (1985). Microbiol. Rev.. 49, 181.
    3. Claus, T. and Akbari, T. unpublished observation.

    Supporting Documents

    Material Safety Datasheets

    The following is a list of Material Safety Data Sheets (MSDS) that apply to this product to help you use it safely. The following file naming structure is used to name these document files: [Product Name] MSDS. For international versions please contact us at info@neb.com.

    Datacards

    The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.
    1. There are several factors that lead to poor results for PFG markers. Here is a list of recommendations for ensuring a sharp high quality banding pattern.
    2. Can I use SYBR® with the DNA Ladders from NEB?