pSV40-GLuc Control Plasmid


The pSV40-GLuc Control Plasmid is a mammalian expression vector that encodes the secreted luciferase from the copepod Gaussia princeps as a reporter, under the control of the constitutive SV40 promoter. Gaussia luciferase (GLuc) is a 19 kDa protein encoded by a “humanized “ sequence, and it contains a native signal peptide at the N-terminus that allows it to be secreted from mammalian cells into the cell culture medium (1) There is a multiple cloning site (MCS) upstream of the SV40 promoter.


  • Polylinker MCS: 1–50
  • SV40 promoter: 51-246
  • GLuc coding: 306–863
  • Start codon: 306–308
  • Stop codon: 861–863
  • Signal peptide: 306–356
  • SV40 poly-A site: 890–1111
  • SV40 enhancer: 1117–1363
  • Bacterial replication ori (pMB1): 2269–1681
  • Amp resistance: 3300–2440

Product Source

Isolated from an E. coli strain NEB10b by standard DNA purification procedure.


Advantages and Features


  • Multiple samples can be obtained from the same transfected cells (i.e., before and after experimental treatments or at multiple time points).
  • 90–95% of GLuc activity is found in the cell culture medium, with the remaining 5–10% detectable in cell lysates. This allows flexibility when assaying GLuc along with other co-transfected reporters.
  • The activity of GLuc is high and the GLuc assay is sensitive enough to detect very small amounts of GLuc enzyme activity.
  • GLuc is very stable in the cell culture medium so the GLuc activity detected reflects the amount of GLuc secreted by the transfected cells over a period of several days. GLuc can also be stored at 4°C for several days without any loss in activity.
  • GLuc does not use the same substrate as Cypridina luciferase. Therefore, it is possible to assay both GLuc and CLuc independently in cell culture medium from cells expressing both reporters (2,3).
  • The pSV40-GLuc Control Plasmid can be transfected into cells using any standard transfection and stable cell lines can be established using Neomycin selection.
Recommended Sequencing Primers for pSV40-GLuc Control Plasmid (not available from NEB)
GLuc 3´ End Forward Primer (20-mer) 

GLuc 5´ End Reverse Primer (24-mer) 


  • The pSV40-GLuc Control Plasmid can be used as a control for assessing the efficiency of transfection in mammalian cells. Plasmids containing other constitutive promoter elements are also available (see Companion Products Sold Separately).
  • GLuc can be used as a stand alone reporter or in conjunction with other compatible reporters such as Cypridina luciferase (CLuc) (2). GLuc and CLuc are ideally suited for co-expression as both are secreted and highly active enzymes providing ease of use and sensitivity (2, 3).

Properties and Usage

Storage Temperature


Storage Conditions

10 mM Tris-HCl
pH 7.5 @ 25°C

Sequence Files

Fasta  GenBank  


  1. Tannous, B.A. et al. (2005). Mol. Ther.. 11, 435-443.
  2. Otsuji, et al. (2004). Anal. Biochemistry. 329, 230-237.
  3. Wu, et al. (2007). Biotechniques. 42, 290-292.


  1. Where can I find the sequence of this plasmid?
  2. Can I generate a stable cell line with pSV40-GLuc Control Plasmid?
  3. Can I transfect this plasmid into mammalian cells?
  4. How do I assay for GLuc expression?
  5. Is there another secreted reporter that can be used with GLuc?

Interactive Tools

Safety Data Sheet

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.