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pSV40-GLuc Control Plasmid

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The pSV40-GLuc Control Plasmid is a mammalian expression plasmid that constitutively expresses the secreted Gaussia luciferase (GLuc) under the control of the SV40 early promoter.

  • Moderate constitutive expression of GLuc
  • GLuc can be assayed in the culture media or cell lysate

Ordering Information

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  • 500 μg/ml
    20 μg
    $269.00
  • Product Information
    The pSV40-GLuc Control Plasmid is a mammalian expression vector that encodes the secreted luciferase from the copepod Gaussia princeps as a reporter, under the control of the constitutive SV40 promoter. Gaussia luciferase (GLuc) is a 19 kDa protein encoded by a “humanized “ sequence, and it contains a native signal peptide at the N-terminus that allows it to be secreted from mammalian cells into the cell culture medium (1) There is a multiple cloning site (MCS) upstream of the SV40 promoter.

    Highlights

    • Polylinker MCS: 1–50
    • SV40 promoter: 51-246
    • GLuc coding: 306–863
    • Start codon: 306–308
    • Stop codon: 861–863
    • Signal peptide: 306–356
    • SV40 poly-A site: 890–1111
    • SV40 enhancer: 1117–1363
    • Bacterial replication ori (pMB1): 2269–1681
    • Amp resistance: 3300–2440

    Product Source

    Isolated from an E. coli strain NEB10b by standard DNA purification procedure.
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    Product Categories:
    Gaussia Luciferase Products
    Applications:
    Gaussia Luciferase,
    DNA Ligation
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